Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
RAT CENTRAL ORL-1 RECEPTOR UNCOUPLES FROM ADENYLYL-CYCLASE DURING MEMBRANE PREPARATION
Autore:
OKAWA H; HIRST RA; SMART D; MCKNIGHT AT; LAMBERT DG;
Indirizzi:
UNIV LEICESTER,LEICESTER ROYAL INFIRM,DEPT ANAESTHESIA LEICESTER LE1 5WW LEICS ENGLAND UNIV LEICESTER,LEICESTER ROYAL INFIRM,DEPT ANAESTHESIA LEICESTER LE1 5WW LEICS ENGLAND PARKE DAVIS NEUROSCI RES CTR CAMBRIDGE CB2 2QB ENGLAND
Titolo Testata:
Neuroscience letters
fascicolo: 1, volume: 246, anno: 1998,
pagine: 49 - 52
SICI:
0304-3940(1998)246:1<49:RCORUF>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
ORPHANIN-FQ; ORL(1) RECEPTOR; OPIOID PEPTIDE; NOCICEPTIN; INHIBITION; CLONING; RELEASE; MEMBER; FAMILY; LOCALIZATION;
Keywords:
ORPHAN OPIOID RECEPTOR; NOCICEPTIN; CANNABINOIDS; RAT BRAIN; CYCLIC ADENOSINE MONOPHOSPHATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
H. Okawa et al., "RAT CENTRAL ORL-1 RECEPTOR UNCOUPLES FROM ADENYLYL-CYCLASE DURING MEMBRANE PREPARATION", Neuroscience letters, 246(1), 1998, pp. 49-52

Abstract

Nociceptin/orphanin FQ is the endogenous agonist of the orphan receptor ORL-1. In this study, we sought to examine any possible regional differences of nociceptin binding using [I-125]Tyr(14)-nociceptin, and of agonist induced inhibition of CAMP formation in membranes prepared from cerebrocortex, cerebellum and brainstem. The binding of [I-125]Tyr(14)-nociceptin was concentration-dependent and saturable, with B-max and pK(d) (pM) values of 179.7 +/- 15.3 fmol/mg protein and 10.26 +/- 0.09 (60.0), 12.4 +/- 1.8 fmol/mg protein and 10.44 +/- 0.07 (37.0), 52.3 +/- 0.8 fmol/mg protein and 10.16 +/- 0.08 (74.0) in cerebrocortical, cerebella and brainstem membranes, respectively. In all preparations, nociceptin up to 1 mu M failed to inhibit basal and forskolin stimulated CAMP formation. In all tissues forskolin stimulated and nabilone (acting at the central cannabinoid receptor) inhibited CAMP formation. Collectively these data report regional differences in ORL-1 receptor expression and that these receptors uncouple during membrane preparation. (C) 1998 Elsevier Science Ireland Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 20:07:47