Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
SALVAGE ANGIOGENESIS INDUCED BY ADENOVIRUS-MEDIATED GENE-TRANSFER OF VASCULAR ENDOTHELIAL GROWTH-FACTOR PROTECTS AGAINST ISCHEMIC VASCULAR OCCLUSION
Autore:
MACK CA; MAGOVERN CJ; BUDENBENDER KT; PATEL SR; SCHWARZ EA; ZANZONICO P; FERRIS B; SANBORN T; ISOM OW; CRYSTAL RG; ROSENGART TK;
Indirizzi:
CORNELL UNIV,MED CTR,NEW YORK HOSP,DEPT CARDIOTHORAC SURG,525 E 68TH ST,F2100 NEW YORK NY 10021 CORNELL UNIV,MED CTR,NEW YORK HOSP,DEPT CARDIOTHORAC SURG NEW YORK NY10021 CORNELL UNIV,MED CTR,NEW YORK HOSP,DIV PULM & CRIT CARE MED NEW YORK NY 10021 CORNELL UNIV,MED CTR,NEW YORK HOSP,DIV NUCL MED NEW YORK NY 10021 CORNELL UNIV,MED CTR,NEW YORK HOSP,DIV CARDIOL NEW YORK NY 10021
Titolo Testata:
Journal of vascular surgery
fascicolo: 4, volume: 27, anno: 1998,
pagine: 699 - 709
SICI:
0741-5214(1998)27:4<699:SAIBAG>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
REPLICATION-DEFICIENT; IN-VIVO; BLOOD-FLOW; THERAPEUTIC ANGIOGENESIS; COLLATERAL DEVELOPMENT; CYSTIC-FIBROSIS; FACTOR FAMILY; UP-REGULATION; EXPRESSION; MYOCARDIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
C.A. Mack et al., "SALVAGE ANGIOGENESIS INDUCED BY ADENOVIRUS-MEDIATED GENE-TRANSFER OF VASCULAR ENDOTHELIAL GROWTH-FACTOR PROTECTS AGAINST ISCHEMIC VASCULAR OCCLUSION", Journal of vascular surgery, 27(4), 1998, pp. 699-709

Abstract

Purpose: Vascular endothelial growth factor (VEGF) is a potent stimulator of angiogenesis, and transgene expression from adenovirus vectorscan provide in vivo delivery of proteins. On the basis of this knowledge, we hypothesized that local administration of a replication-deficient adenovirus vector expressing complementary DNA for VEGF (AdVeGF) would induce collateral vessel formation in the setting of ischemia that could protect against subsequent acute vascular occlusion. Methods: Hindlimb ischemia was induced in Sprague-Dawley rats by means of unilateral ligation of the common iliac artery immediately followed by administration of 4 x 10(9)-plaque-forming units VEGF, the control vector AdNull, or phosphate-buffered saline solution into the iliofemoral adipose tissue and thigh muscles. Untreated rats with common iliac ligation were used as an additional control group. Results: Local VEGF expression was observed for 5 days in AdVEGF-treated rats but not in controls. Three weeks after ligation and vector administration, the ipsilateral femoral artery was ligated for a model of an acute vascular occlusion in the setting of preexisting ischemia. Blood flow to the ischemichindlimb relative to the contralateral hindlimb evaluated with color microspheres demonstrated significantly increased blood now in the AdVEGF-treated rats compared with each control group (P < 0.0001). Relative blood now assessed by means of Tc-99m-sestamibi radionuclide scans also demonstrated increased blood flow to the ligated hindlimb of AdVEGF-treated rats compared with each control group (p < 0.002). AdVEGF-treated rats also demonstrated increased vascularity in the ligated limb compared with each control group as assessed by means of angiography(p < 0.0001) and histologic quantification of blood vessels less than80 mu m diameter in local adipose tissue and capillaries per muscle fiber (p < 0.0002). AdVEGF treatment prevented a rise in femoral venouslactate femoral venous concentrations 1 hour after femoral artery ligation in control rats (p < 0.04). Conclusions: An adenovirus vector expressing VEGF complementary DNA is capable of stimulating an angiogenic response that protects against acute vascular occlusion in the setting of preexisting ischemia, suggesting that in vivo gene transfer of VEGE complementary DNA might be useful in prophylaxis of advancing arterial occlusive disease.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/10/20 alle ore 00:43:21