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Titolo:
RAPID IN-VITRO PROPAGATION OF EAST-INDIAN ROSEWOOD (DALBERGIA-LATIFOLIA ROXB.) THROUGH HIGH-FREQUENCY SHOOT PROLIFERATION FROM COTYLEDONARYNODES
Autore:
PRADHAN C; PATTNAIK S; CHAND PK;
Indirizzi:
UTKAL UNIV,PG DEPT BOT,PLANT TISSUE & CELL CULTURE FACIL BHUBANESWAR 751004 ORISSA INDIA UTKAL UNIV,PG DEPT BOT,PLANT TISSUE & CELL CULTURE FACIL BHUBANESWAR 751004 ORISSA INDIA
Titolo Testata:
Journal of Plant Biochemistry and Biotechnology
fascicolo: 1, volume: 7, anno: 1998,
pagine: 61 - 64
SICI:
0971-7811(1998)7:1<61:RIPOER>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
TISSUE-CULTURE; REGENERATION; MICROPROPAGATION; PLANTLETS; CALLUS; TREES;
Keywords:
COTYLEDONARY NODE; DALBERGIA LATIFOLIA; IN VITRO PROPAGATION; SHOOT PROLIFERATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
16
Recensione:
Indirizzi per estratti:
Citazione:
C. Pradhan et al., "RAPID IN-VITRO PROPAGATION OF EAST-INDIAN ROSEWOOD (DALBERGIA-LATIFOLIA ROXB.) THROUGH HIGH-FREQUENCY SHOOT PROLIFERATION FROM COTYLEDONARYNODES", Journal of Plant Biochemistry and Biotechnology, 7(1), 1998, pp. 61-64

Abstract

An efficient protocol is described for large scale in vitro propagation of east Indian rosewood (Dalbergia latifolia Roxb.) using cotyledonary nodes derived from axenic seedlings. Of the four different cytokinins tested, BA was most effective in inducing multiple shoot buds in the explants. High frequency shoot proliferation (93%) coupled with maximum number of shoot formation (10-12 shoots/explant) was recorded on Murashige and Skoog's medium supplemented with 2.0 mg/l BA. The frequency of shoot proliferation declined at higher levels of cytokinins. Shoot culture was multiplied by subculturing the original cotyledonary node on a fresh shoot multiplication medium each time after excising the newly formed shoots. Shoots obtained from each passage were multiplied further as nodal explants and each node produced 3-4 shoots. In twomonths from a single cotyledonary node, about 90 shoots were obtained. Rooting was induced in 72% of the regenerated shoots on half-strength MS containing IAA, IBA and IPA each at 1.0 mg/l. Rooted plantlets were hardened off and eventually established in soil.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 10:54:34