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Titolo:
TOXICITY OF POLYUNSATURATED FATTY-ACID ESTERS FOR HUMAN MONOCYTE-MACROPHAGES - THE ANOMALOUS BEHAVIOR OF CHOLESTERYL LINOLENATE
Autore:
HARDWICK SJ; CARPENTER KLH; LAW NS; VANDERVEEN C; MARCHANT CE; HIRD R; MITCHINSON MJ;
Indirizzi:
UNIV CAMBRIDGE,DEPT PATHOL,DIV CELLULAR PATHOL,TENNIS COURT RD CAMBRIDGE CB2 1QP ENGLAND
Titolo Testata:
Free radical research
fascicolo: 4, volume: 26, anno: 1997,
pagine: 351 - 362
SICI:
1071-5762(1997)26:4<351:TOPFEF>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; HUMAN ATHEROSCLEROTIC LESIONS; OXIDIZED LIPIDS; CYTO-TOXICITY; OXIDATION; INVITRO; LINOLEATE; ALDEHYDES; DISEASE; DAMAGE;
Keywords:
CYTOTOXICITY; OXIDATION; POLYUNSATURATED FATTY ACID ESTERS; LINOLENATE; MONOCYTE-MACROPHAGES (HUMAN); ATHEROSCLEROSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
S.J. Hardwick et al., "TOXICITY OF POLYUNSATURATED FATTY-ACID ESTERS FOR HUMAN MONOCYTE-MACROPHAGES - THE ANOMALOUS BEHAVIOR OF CHOLESTERYL LINOLENATE", Free radical research, 26(4), 1997, pp. 351-362

Abstract

We have investigated the toxicity to human manocyte-macrophages, and susceptibility to oxidation, of different individual dietary fatty acids in cholesterol esters and triglycerides, added to the cell culturesas coacervates with bovine serum albumin. Toxicity was assessed usingrelease of radioactivity from cells preloaded with tritiated adenine. Lipid oxidation was measured by gas chromatography (GC). The triglycerides showed a direct relationship between toxicity and increasing unsaturation, which in turn correlated with increasing susceptibility to oxidation. Triolein (18:1; omega-9) and trilinolein (18:2; omega-6) were non-toxic. Trilinolenin (18:3; omega-3) was toxic only after prolonged incubation. Triarachidonin (20:4; omega-6), tridocosapentaenoin (20:5; omega-3) and tridocosahexaenoin (22:6; omega-3) were profoundly and rapidly toxic. There was a similar relationship between toxicity and increasing unsaturation for most of the cholesterol esters, but cholesteryl linolenate was apparently anomalous, being non-toxic in spite of possessing three double bonds and being extensively oxidised. Probucol and DL-a-tocopherol conferred protection against the toxicity of cholesteryl arachidonate and triarachidonin. The oxidation in these experiments was largely independent of the presence of cells. GC indicated that formation of 7-oxysterols might contribute to the toxicity of cholesteryl linoleate. The toxicity of triglycerides suggests that polyunsaturated fatty acid peroxidation products are also toxic. Possible mechanisms of cytotoxicity and relevance to atherosclerosis are discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/02/20 alle ore 14:34:51