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Titolo:
INTERLEUKIN-2 INDUCES DEVELOPMENT OF DENDRITIC CELLS FROM CORD-BLOOD CD34(+) CELLS
Autore:
BYKOVSKAJA SN; BUFFO MJ; BUNKER M; ZHANG HF; MAJORS A; LOKSHIN A; LEVITT ML; JAJA A; SCALISE D; KOSIBAN D; EVANS C; MARKS S; SHOGAN J;
Indirizzi:
320 E N AVE PITTSBURGH PA 15212 ALLEGHENY UNIV HLTH SCI,DEPT LAB MED CARDIOVASC RES,LUNG CANC PROGRAMHUMAN GENET PITTSBURGH PA 00000 ALLEGHENY UNIV HLTH SCI,RADIAT ONCOL ALLEGHENY SINGER RES INST PITTSBURGH PA 00000
Titolo Testata:
Journal of leukocyte biology
fascicolo: 5, volume: 63, anno: 1998,
pagine: 620 - 630
SICI:
0741-5400(1998)63:5<620:IIDODC>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
NATURAL-KILLER-CELLS; COLONY-STIMULATING FACTOR; NECROSIS-FACTOR-ALPHA; HEMATOPOIETIC PROGENITOR CELLS; C-KIT-LIGAND; BONE-MARROW; LANGERHANS CELLS; STEM-CELL; NK CELLS; COSTIMULATORY MOLECULE;
Keywords:
HEMATOPOIETIC CYTOKINES; MAJOR HISTOCOMPATIBILITY COMPLEX II; NATURAL KILLER CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
S.N. Bykovskaja et al., "INTERLEUKIN-2 INDUCES DEVELOPMENT OF DENDRITIC CELLS FROM CORD-BLOOD CD34(+) CELLS", Journal of leukocyte biology, 63(5), 1998, pp. 620-630

Abstract

Dendritic cells (DC) have been shown to develop, along a myeloid or lymphoid lineage of differentiation propagated from bone marrow or early thymic precursor cells with hematopoietic cytokines. In our study, we have induced growth and differentiation of DC from cord blood CD34(+) cells initiated in interleukin-2 (IL-2) alone or in IL-2 + stem cellfactor (SCP) + tumor necrosis factor alpha (TNF-alpha)-supplemented medium and cultured with IL-2 or IL-2 + SCF for 28-35 days, Dendritic morphology and antigenic phenotype of DC grown with IL-2 were characteristic for DC cultured in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF). Growth and differentiation of DC was followed by an increase in expression of MHC II and co-stimulating molecules CD80 and CD86. We have also shown the expression of the IL-2 receptor (IL-2R) gamma-chain in CD34(+) cells after 2-3 days of culture with IL-2 alone. The co-expression of the IL-2R alpha, beta, and gamma subunits in both DC cultured with IL-2- or GM-CSF-containing cocktail ofcytokines was also shown. The time curve for induction of IL-2R demonstrated low levels of subunit expression at the beginning of culture, The number of CD1a cells co-expressing CD25, CD122 and CD gamma increased to about 24-693 and to 78-95% after 21 and 28-35 days, respectively. Development of natural killer cells was shown along with DC. The proportion of CD56(+) cells and cytotoxicity increased in a time-dependent manner.

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Documento generato il 19/09/20 alle ore 21:12:25