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Titolo:
MORPHOLOGICAL-STUDIES OF GLOMERULI IN OBSTRUCTIVE KIDNEYS BY CONFOCALLASER-SCANNING MICROSCOPY AND QUICK-FREEZING REPLICA METHOD
Autore:
MATSUDA A; TERADA N; UEDA H; FUJII Y; TAGO K; UENO A; OHNO S;
Indirizzi:
YAMANASHI MED UNIV,DEPT UROL,1110 SHIMOKATO TAMAHO YAMANASHI 40938 JAPAN YAMANASHI MED UNIV,DEPT ANAT TAMAHO YAMANASHI 40938 JAPAN
Titolo Testata:
Histology and histopathology
fascicolo: 2, volume: 13, anno: 1998,
pagine: 337 - 346
SICI:
0213-3911(1998)13:2<337:MOGIOK>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEEP-ETCHING METHOD; YOUNG-RATS; UNINEPHRECTOMY; ULTRASTRUCTURE; NEPHROPATHY; HYALINOSIS; SCLEROSIS; NEPHRITIS; DAMAGE; RABBIT;
Keywords:
PODOCYTE; CYTOSKELETON; OBSTRUCTIVE KIDNEY; CONFOCAL LASER MICROSCOPY; QUICK-FREEZING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
A. Matsuda et al., "MORPHOLOGICAL-STUDIES OF GLOMERULI IN OBSTRUCTIVE KIDNEYS BY CONFOCALLASER-SCANNING MICROSCOPY AND QUICK-FREEZING REPLICA METHOD", Histology and histopathology, 13(2), 1998, pp. 337-346

Abstract

Morphological changes of glomeruli in obstructive kidneys were studied by using confocal laser scanning microscopy (CLSM), and quick-freezing and deep-etching (QF-DE) method. Twenty-one rabbits were divided into three groups, consisting of control, 6-hr bilateral ureteral obstruction (BUO) and 24-hr BUO. In the experimental groups, the attenuationof cell bodies, the lengthening and stretching of major processes, the cystic formation in the cytoplasm and the fusion of foot processes were observed on conventional ultrathin sections. These changes in the 24-hr BUO group were more clearly observed than those in the 6-hr BUO group. By the CLSM, cell bodies and foot processes of podocytes in theexperimental groups were more intensely immunostained with anti-a-tubulin antibody and phalloidin-FITC. By the QF-DE method, cytoskeletons in the podocyte cell bodies and major processes were composed of numerous intermediate filaments, but distinct changes of actin filaments and microtubules were not observed in the control and experimental groups. Considering the physiological changes in BUO, the mechanical stressappeared to be brought about by hemodynamic factors rather than the change of intratubular pressure, resembling the morphological changes in experimental animals with hyperfiltration and the homeostatic adaptation of podocytes under the BUO condition.

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Documento generato il 28/11/20 alle ore 15:32:08