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Titolo:
EFFECTS OF DOPAMINE AND ESTROGEN ON THE REGULATION OF PIT-1-ALPHA, PIT-1-BETA, AND PL-II GENE-EXPRESSION IN THE RAT PLACENTA
Autore:
LEE CK; KANG HS; LEE BJ; KANG HM; CHOI WS; KANG SG;
Indirizzi:
INJE UNIV,COLL NAT SCI,DEPT BIOL KIMHAE 621749 SOUTH KOREA INJE UNIV,COLL NAT SCI,DEPT BIOL KIMHAE 621749 SOUTH KOREA INJE UNIV,INST BASIC SCI KIMHAE 621749 SOUTH KOREA UNIV ULSAN,COLL NAT SCI,DEPT BIOL ULSAN 680749 SOUTH KOREA CHUNGJU UNIV,DEPT GENET ENGN CHUNGJU 360764 SOUTH KOREA GYEONGSANG NATL UNIV,COLL MED,DEPT ANAT CHINJU 6601701 SOUTH KOREA
Titolo Testata:
Molecules and cells
fascicolo: 2, volume: 8, anno: 1998,
pagine: 205 - 211
SICI:
1016-8478(1998)8:2<205:EODAEO>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROLACTIN GENE; GROWTH-HORMONE; TRANSCRIPTION FACTOR; CHORIONIC SOMATOMAMMOTROPIN; RIBONUCLEIC-ACID; LACTOGEN-I; 2 FORMS; PITUITARY; PROMOTER; FAMILY;
Keywords:
DOPAMINE; ESTROGEN; PIT-1-ALPHA, BETA; PL-II; PLACENTA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
C.K. Lee et al., "EFFECTS OF DOPAMINE AND ESTROGEN ON THE REGULATION OF PIT-1-ALPHA, PIT-1-BETA, AND PL-II GENE-EXPRESSION IN THE RAT PLACENTA", Molecules and cells, 8(2), 1998, pp. 205-211

Abstract

Pituitary-specific transcription factor Pit-1 regulates growth hormone and prolactin gene expression in the pituitary, Recently, Pit-1 was shown to be locally synthesized in the rat placenta and is involved inthe regulation of rat placental lactogen (PL) gene expression. Pit-1 has three different splicing variants. They are well known as being biologically active. In the present study, we found that Pit-1 beta is also synthesized in the rat placenta and we tried to examine the effects of dopamine and estrogen on the regulation of Pit-1 alpha, beta and PL-II genes expression using the reverse transcription-polymerase chain reaction (RT-PCR) and Northern blot hybridization, A dopamine receptor agonist, bromocriptine, significantly decreased placental Pit-1 alpha, beta, and PL-II mRNA levels. To examine the effect of estrogen on the gene expression of Pit-1 beta, pregnant female rats were ovariectomized (OVX) and injected daily with 17 beta-estradiol, OVX markedly lowered the amount of Pit-1 beta mRNA, Estrogen injection recovered the OVX-induced inhibition of Pit-1 beta mRNA level, Finally, we investigated the site-specific transcription of Pit-1 alpha and beta mRNA in the labyrinth zone and junctional zone of the placenta in 15 and 20 daysof gestation. The main site of Pit-1 alpha and beta synthesis was shifted from the junctional zone to the labyrinth zone from 15 to 20 daysof gestation. Together, these data presume that Pit-1 beta may play amore important role in the placenta than in the pituitary and that Pit-1 may be involved in the regulation of the PL and prolactin-like peptide by estrogen and dopamine in the rat placenta.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 10:58:15