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Titolo:
NEGATIVE REGULATION OF DNA-REPLICATION BY THE RETINOBLASTOMA PROTEIN IS MEDIATED BY ITS ASSOCIATION WITH MCM7
Autore:
STERNER JM; DEWKNIGHT S; MUSAHL C; KORNBLUTH S; HOROWITZ JM;
Indirizzi:
N CAROLINA STATE UNIV,COLL VET MED,DEPT ANAT PHYSIOL SCI & RADIOL RALEIGH NC 27606 DUKE UNIV,MED CTR,DEPT MOL CANC BIOL DURHAM NC 27710 DUKE UNIV,MED CTR,DEPT MICROBIOL DURHAM NC 27710 DUKE UNIV,MED CTR,DEPT CELL BIOL DURHAM NC 27710 UNIV KONSTANZ,DIV BIOL D-77434 CONSTANCE GERMANY
Titolo Testata:
Molecular and cellular biology
fascicolo: 5, volume: 18, anno: 1998,
pagine: 2748 - 2757
SICI:
0270-7306(1998)18:5<2748:NRODBT>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUSCEPTIBILITY GENE-PRODUCT; SINGLE-STRANDED-DNA; SV40 LARGE-T; CELL-CYCLE; AMINO-TERMINUS; RB PROTEIN; XENOPUS EGGS; MUSCLE-CELLS; G1 PHASE; FAMILY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
89
Recensione:
Indirizzi per estratti:
Citazione:
J.M. Sterner et al., "NEGATIVE REGULATION OF DNA-REPLICATION BY THE RETINOBLASTOMA PROTEIN IS MEDIATED BY ITS ASSOCIATION WITH MCM7", Molecular and cellular biology, 18(5), 1998, pp. 2748-2757

Abstract

A yeast two-hybrid screen was employed to identify human proteins that specifically bind the amino-terminal 400 amino acids of the retinoblastoma (Rb) protein. Two independent cDNAs resulting from this screen were found to encode the carboxy-terminal 137 amino acids of MCM7, a member of a family of proteins that comprise replication licensing factor. Full-length Rb and MCM7 form protein complexes in vitro, and the amino termini of two Rb-related proteins, p107 and p130, also bind MCM7. Protein complexes between Rb and MCM7 were also detected in anti-Ph immunoprecipitates prepared from human cells. The amino-termini of Rb and p130 strongly inhibited DNA replication in an MCM7-dependent fashion in a Xenopus in vitro DNA replication assay system. These data pro,ide the first evidence that Rb and Ph-related proteins can directly regulate DNA replication and that components of licensing factor are targets of the products of tumor suppressor genes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 04:08:30