Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
REARRANGEMENT OF THE PH-20 PROTEIN ON THE SURFACE OF MACAQUE SPERMATOZOA FOLLOWING EXPOSURE TO ANTI-PH-20 ANTIBODIES OR BINDING TO ZONA-PELLUCIDA
Autore:
YUDIN AI; CHERR GN; VANDEVOORT CA; OVERSTREET JW;
Indirizzi:
UNIV CALIF DAVIS,INST TOXICOL & ENVIRONM HLTH,DIV REPROD BIOL,DEPT OBSTET & GYNECOL DAVIS CA 95616 UNIV CALIF DAVIS,INST TOXICOL & ENVIRONM HLTH,DIV REPROD BIOL,DEPT OBSTET & GYNECOL DAVIS CA 95616 UNIV CALIF DAVIS,BODEGA MARINE LAB DAVIS CA 95616 UNIV CALIF DAVIS,CALIF REG PRIMATE RES CTR DAVIS CA 95616
Titolo Testata:
Molecular reproduction and development
fascicolo: 2, volume: 50, anno: 1998,
pagine: 207 - 220
SICI:
1040-452X(1998)50:2<207:ROTPPO>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPERM PLASMA-MEMBRANE; ACROSOME REACTION; MOUSE SPERM; HYALURONIDASE ACTIVITY; MAMMALIAN SPERM; CROSS-LINKING; EGG; HAMSTER; FERTILIZATION; ANTIGEN;
Keywords:
MACAQUE SPERM; PH-20; ZONA PELLUCIDA; ACROSOME REACTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
54
Recensione:
Indirizzi per estratti:
Citazione:
A.I. Yudin et al., "REARRANGEMENT OF THE PH-20 PROTEIN ON THE SURFACE OF MACAQUE SPERMATOZOA FOLLOWING EXPOSURE TO ANTI-PH-20 ANTIBODIES OR BINDING TO ZONA-PELLUCIDA", Molecular reproduction and development, 50(2), 1998, pp. 207-220

Abstract

Capacitated cynomolgus macaque sperm have a surface hyaluronidase (PH-20) that is evenly distributed over the entire head and can be visualized at the ultrastructural level using a secondary antibody labeled with colloidal gold. Exposure of sperm to mono-specific, bivalent polyclonal antibodies to PH-20 causes a rapid clustering of PH-20. The predominant morphological consequence of PH-20 redistribution is its aggregation along the lateral edge of the sperm head. Monovalent Fab fragments of the anti-PH-20 antibody bound to the sperm head but did not induce a change in PH-20 distribution. PH-20 aggregation was observed in almost all sperm following treatment with the polyclonal antibody, butonly about 20% of the sperm had morphological acrosome reactions, regardless of the time of exposure or the concentration of antibody. There was morphological evidence of swelling of the acrosomal matrix in over 50% of the sperm following exposure to anti-PH-20 antibodies. Anti-PH-20 Fab fragments did not induce the acrosome reaction or acrosomal matrix swelling. Sperm bound to macaque zona pellucida also showed aggregation of the PH-20 protein as soon as 30 sec after sperm-zona interaction. This aggregation was not observed when macaque sperm were bound to hamster zona pellucida. When macaque sperm were surface-labeled with biotin and then incubated with anti-PH-20 antibodies or macaque zona pellucida, there was no evidence of a global surface protein rearrangement, although PH-20 protein was aggregated on the surface of the same sperm cells. An increase in levels of internal sperm Ca++ was measured in association with the antibody-induced PH-20 aggregation. Fab fragments did not increase Ca++ levels, but when they were crosslinked with anti-fab antibody there was a significant Ca++ increase and induction of acrosome reactions. Anti-PH-20 Fab fragments did not block macaque sperm binding to macaque zona pellucida or the zona-induced acrosome reaction. We conclude that PH-20 on the sperm surface is involved in sperm-zona pellucida interaction and the zona-induced acrosome reaction. (C) 1998 Wiley-iiss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 13:39:50