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Titolo:
DETERMINATION OF TUMOR-CELL PROCOAGULANT ACTIVITY BY SONOCLOT(TM) ANALYSIS IN WHOLE-BLOOD
Autore:
AMIRKHOSRAVI A; BIGGERSTAFF JP; WARNES G; FRANCIS DA; FRANCIS JL;
Indirizzi:
FLORIDA HOSP,WALT DISNEY MEM CANC INST,HEMOSTASIS & THROMBOSIS RES UNIT,616 E ALTAMONTE DR ALTAMONTE SPRINGS FL 32701 FLORIDA HOSP,WALT DISNEY MEM CANC INST,HEMOSTASIS & THROMBOSIS RES UNIT ALTAMONTE SPRINGS FL 32701
Titolo Testata:
Thrombosis research
fascicolo: 5, volume: 84, anno: 1996,
pagine: 323 - 332
SICI:
0049-3848(1996)84:5<323:DOTPAB>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDUCED PLATELET-AGGREGATION; THROMBOSIS-AND-HEMOSTASIS; X-ACTIVATING PROCOAGULANT; BREAST-CANCER-CELLS; TISSUE FACTOR; IN-VITRO; STANDARDIZATION COMMITTEE; MALIGNANT DISEASE; COAGULATION; ABNORMALITIES;
Keywords:
COAGULATION; CANCER; TISSUE FACTOR; SONOCLOT(TM) ANALYSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
A. Amirkhosravi et al., "DETERMINATION OF TUMOR-CELL PROCOAGULANT ACTIVITY BY SONOCLOT(TM) ANALYSIS IN WHOLE-BLOOD", Thrombosis research, 84(5), 1996, pp. 323-332

Abstract

Coagulation activation in cancer may be due to expression of procoagulant activity (PCA) by tumor cells. Some PCA activate coagulation, while others influence platelet aggregation. Thus, clotting time assessments of PCA may not reflect the potential for hypercoagulability. We therefore studied the effect of various malignant and nonmalignant cellson whole blood coagulation using the Sonoclot(TM) Analyzer. Five human (HT29 colon, 582 bladder, MCF-7 breast, BT-474 breast and A375 malignant melanoma) and three rodent (MC28, WEHI-164 and Neuro2a) cell lines were used. Rat thymocytes and peritoneal macrophages and human endotoxin-stimulated mononuclear cells and umbilical vein endothelial cells(HUVEC) were used as non-malignant controls. All tumor cells markedlyshortened the recalcification time of citrated blood and the most potent (HT29 and J82) also increased clot rate (P <0.01). The breast cancer cells MCF-7 and BT-474 expressed only weak PCA and did not affect clotting rate. None of the nonmalignant cells significantly affected clotting time or rate in whole blood. J82 and HT29 cells grown in serum-rich media shortened the recalcification time of both normal and FVII-deficient plasmas. However, cells grown in serum-free conditions had significantly less PCA in FVII-deficient plasma. We conclude that the Sonoclot(TM) Analyzer is useful for determining cellular PCA; significant PCA is a feature of malignant cells and cells grown in medium containing serum supplements cannot be reliably evaluated for PCA. Copyright (C) 1996 Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 10:08:12