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Titolo:
VECTORIAL SECRETION OF VASCULAR ENDOTHELIAL GROWTH-FACTOR BY POLARIZED HUMAN ENDOMETRIAL EPITHELIAL-CELLS
Autore:
HORNUNG D; LEBOVIC DI; SHIFREN JL; VIGNE JL; TAYLOR RN;
Indirizzi:
UNIV CALIF SAN FRANCISCO,DEPT OBSTET GYNECOL & REPROD SCI,CTR REPROD ENDOCRINOL SAN FRANCISCO CA 94143 UNIV CALIF SAN FRANCISCO,DEPT OBSTET GYNECOL & REPROD SCI,CTR REPROD ENDOCRINOL SAN FRANCISCO CA 94143
Titolo Testata:
Fertility and sterility
fascicolo: 5, volume: 69, anno: 1998,
pagine: 909 - 915
SICI:
0015-0282(1998)69:5<909:VSOVEG>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
MENSTRUAL-CYCLE; IN-VITRO; LOCALIZATION; RECEPTOR; IDENTIFICATION; ANGIOGENESIS; EXPRESSION; GENE; RAT;
Keywords:
ANGIOGENESIS; IMPLANTATION; MITOGEN; PREGNANCY; UTERUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
D. Hornung et al., "VECTORIAL SECRETION OF VASCULAR ENDOTHELIAL GROWTH-FACTOR BY POLARIZED HUMAN ENDOMETRIAL EPITHELIAL-CELLS", Fertility and sterility, 69(5), 1998, pp. 909-915

Abstract

Objective: To analyze directional vascular endothelial growth factor (VEGF) secretion in polarized human endometrial epithelial cell cultures. VEGF has distinct distribution patterns in human endometrium. Stromal cells are diffusely positive for VEGF messenger RNA and protein, whereas glandular epithelium shows focal VEGF immunostaining at the apical surface. The epithelial distribution suggests that VEGF is secreted into gland lumina, potentially influencing the nutrition and/or apposition of the developing blastocyst. Design: Controlled in vitro studyof protein secretion by polarized endometrial epithelial cells established on polyethylene filters. Setting: University hospital. Patient(s): Endometrial biopsies were obtained from healthy, ovulatory women undergoing elective surgery. Intervention(s): Primary endometrial epithelial cells were cultured. Main Outcome Measure(s): VEGF mRNA and protein production were measured in polarized cells. The secretion of VEGF was determined. Result(s): VEGF production by endometrial epithelial cells was verified by Northern blotting and immunoassays of conditionedmedia. The mean (+/-SD) apical secretion of VEGF was 3.9 +/- 1.4 ng per 10(5) cells every 48 hours and the mean (+/-SD) basal secretion was0.8 +/- 0.2 ng per 10(5) cells every 48 hours. In contrast, the apical and basal secretion of a soluble cellular isoform of fibronectin were 2.76 +/- 0.96 ng per 10(5) cells every 48 hours and 2.64 +/- 1.79 ngper 10(5) cells every 48 hours, respectively. Conclusion(s): VEGF is secreted preferentially into the lumina of endometrial glands. Apical VEGF may be an endometrial signal for blastocyst development or implantation. (C) 1998 by American Society for Reproductive Medicine.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 14:29:40