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Titolo:
EFFECTS OF BRIEF GLUCOCORTICOID EXPOSURE ON GROWTH OF VASCULAR SMOOTH-MUSCLE CELLS IN CULTURE
Autore:
KAWAI Y; HAYASHI T; EGUCHI K; ASAZUMA K; MASAMURA K; IWAMURO A; TAKANO Y; TADA H; MATSUKAWA S; MIYAMORI I;
Indirizzi:
FUKUI MED UNIV,DEPT INTERNAL MED 3 FUKUI 9101104 JAPAN FUKUI MED UNIV,CENT RES LABS FUKUI 9101104 JAPAN
Titolo Testata:
Biochemical and biophysical research communications
fascicolo: 2, volume: 245, anno: 1998,
pagine: 493 - 496
SICI:
0006-291X(1998)245:2<493:EOBGEO>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
ATHEROSCLEROSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
13
Recensione:
Indirizzi per estratti:
Citazione:
Y. Kawai et al., "EFFECTS OF BRIEF GLUCOCORTICOID EXPOSURE ON GROWTH OF VASCULAR SMOOTH-MUSCLE CELLS IN CULTURE", Biochemical and biophysical research communications, 245(2), 1998, pp. 493-496

Abstract

While prolonged exposure of vascular smooth muscle cells (VSMC) to glucocorticoid has been shown to inhibit cell proliferation, the effect of a brief pulse exposure is not known. We studied the short-term effects of pulse exposure to dexamethasone (DEX) on DNA synthesis in cultured VSMC, VSMC were pulsed with DEX for varying time intervals and [H-3]thymidine incorporation into cells after 24 h was measured. Exposureto DEX for 24 h decreased [H-3]thymidine incorporation, while pulse treatments with DEX from 2 min to 6 h significantly increased [H-3]thymidine incorporation. Maximal proliferative effect was observed with a 20-min exposure. The effect of a 20-min pulse was dose-dependent, withthe half-maximal dose of DEX being approximately 10(-7) M. A selective glucocorticoid re ceptor antagonist, RU486, inhibited the proliferative effect of DEX, Concentrated conditioned medium from cells exposed to 10(-6) M DEX increased [H-3]thymidine incorporation by other VSMC in a dose-dependent manner. These results suggest that short term pulseDEX exposure is capable of producing one or more autocrine growth factors in VSMC via a glucocorticoid receptor action. This effect of glucocorticoid pulses may contribute to the pathogenesis of arteriosclerosis and hypertension. (C) 1998 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 23:45:15