Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
REGULATION OF TRANSCRIPTION OF THE TATA-LESS HUMAN-COMPLEMENT COMPONENT C4 GENE
Autore:
VAISHNAW AK; MITCHELL TJ; ROSE SJ; WALPORT MJ; MORLEY BJ;
Indirizzi:
HAMMERSMITH HOSP,IMPERIAL COLL SCI TECHNOL & MED,DIV MED,RHEUMATOL SECT,DU CANE RD LONDON W12 0NN ENGLAND HAMMERSMITH HOSP,IMPERIAL COLL SCI TECHNOL & MED,DIV MED,RHEUMATOL SECT LONDON W12 0NN ENGLAND IMPERIAL COLL SCH MED,DIV MED,RHEUMATOL SECT LONDON ENGLAND
Titolo Testata:
The Journal of immunology
fascicolo: 9, volume: 160, anno: 1998,
pagine: 4353 - 4360
SICI:
0022-1767(1998)160:9<4353:ROTOTT>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA POLYMERASE-II; BOX-BINDING-PROTEINS; START SITE LOCATION; PROMOTER ELEMENTS; INTERFERON-GAMMA; EPITHELIAL-CELLS; MULTIPLE FORMS; MOLECULAR MAP; GLOBIN GENE; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
A.K. Vaishnaw et al., "REGULATION OF TRANSCRIPTION OF THE TATA-LESS HUMAN-COMPLEMENT COMPONENT C4 GENE", The Journal of immunology, 160(9), 1998, pp. 4353-4360

Abstract

The 5'-sequences flanking the human complement component C4 genes (C4A and C4B) have been analyzed for their ability to direct expression of a reporter gene in cell lines that constitutively express or do not express C4, No difference in the level of reporter gene expression wasdetected in cells transfected with C4A- or C4B-specific constructs. Aseries of reporter constructs containing progressively truncated C4 promoter fragments transfected into the hepatocyte Hep G2 cell line, identified the sequence contained within the region -178 to -39 as that associated with maximal reporter gene expression. This region containsconsensus binding moths for nuclear factor 1 (-110 to -97), Sp1 (-57 to -49), and three basic helix-loop-helix (-137 to -132, -98 to -93, and -78 to -73)-like transcription factors. Electromobility shift assays and DNase I footprinting analysis showed specific DNA-protein interactions of the C4 promoter at the nuclear factor 1, two E box (-98 to -93 and -78 to -73), and Spl binding domains. Site-directed mutagenesisof the Spl binding site resulted in total abrogation of reporter geneexpression and mutation of the E box (-78 to -73) resulted in a 8-fold reduction in expression. We conclude that the Spl binding site at position -57 to -49 is critical for accurately initiated, basal transcription of C4.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 13:06:23