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Titolo:
ANALYSIS OF ANTIGENS RECOGNIZED BY AUTOANTIBODIES IN HERPES-GESTATIONIS - USEFULNESS OF IMMUNOBLOTTING USING A FUSION PROTEIN REPRESENTING AN EXTRACELLULAR DOMAIN OF THE 180 KD BULLOUS PEMPHIGOID ANTIGEN
Autore:
MURAKAMI H; AMAGAI M; HIGASHIYAMA M; HASHIMOTO K; CHORZELSKI TP; BHOGAL BS; JENKINS RE; BLACK MM; ZILLIKENS D; NISHIKAWA T; HASHIMOTO T;
Indirizzi:
KEIO UNIV,SCH MED,DEPT DERMATOL,SHINJUKU KU,35 SHIMANOMACHI TOKYO 160JAPAN KEIO UNIV,SCH MED,DEPT DERMATOL,SHINJUKU KU TOKYO 160 JAPAN OSAKA UNIV,SCH MED,DEPT DERMATOL SUITA OSAKA 565 JAPAN KLIN DERMATOL AM PL-02008 WARSAW POLAND ST THOMAS HOSP,ST JOHNS INST DERMATOL,DEPT DERMATOPATHOL LONDON SE1 7EH ENGLAND UNIV WURZBURG,DEPT DERMATOL D-97080 WURZBURG GERMANY
Titolo Testata:
Journal of dermatological science
fascicolo: 2, volume: 13, anno: 1996,
pagine: 112 - 117
SICI:
0923-1811(1996)13:2<112:AOARBA>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
BMZ ANTIBODIES; HG-FACTOR; BP180; IMMUNOFLUORESCENCE; IMMUNOPATHOLOGY; CLONING;
Keywords:
AUTOANTIGEN; BULLOUS PEMPHIGOID; HERPES GESTATIONIS; IMMUNOBLOTTING; FUSION PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
H. Murakami et al., "ANALYSIS OF ANTIGENS RECOGNIZED BY AUTOANTIBODIES IN HERPES-GESTATIONIS - USEFULNESS OF IMMUNOBLOTTING USING A FUSION PROTEIN REPRESENTING AN EXTRACELLULAR DOMAIN OF THE 180 KD BULLOUS PEMPHIGOID ANTIGEN", Journal of dermatological science, 13(2), 1996, pp. 112-117

Abstract

Herpes gestationis (HG) is a ran pregnancy-associated disease. The aim of this study was to compare various immunohistochemical and immunobiochemical techniques with respect to their diagnostic sensitivity forHG. We studied 43 HG sera; only half of these reacted with the basement membrane zone (BMZ) with both indirect immunofluorescence (IF) and complement IF of normal human skin. 81% of the sera reacted with the epidermal side of I M NaCl-split skin. In general, titers of anti-BMZ antibodies in HG sera were lower than those in bullous pemphigoid (BP) sera. Immunoblot analysis of human epidermal extracts showed that 51% of HG sera recognized the 180 kD BP antigen (BP180) and 26% recognizedthe 230 kD BP antigen (BP230). We also studied the reactivity of HG sera with fusion proteins representing either the NC16a domain of humanBP180 or the C-terminal region of mouse BP230. Whereas 79% of HG serareacted with the BP180 fusion protein, only 5% recognized the BP230 fusion protein. Our results suggest that indirect IF of 1 M NaCl-split skin and immunoblotting of a fusion protein representing the BP180 NC16a domain are more sensitive techniques for the diagnosis of HG than conventional and complement IF or immunoblotting of crude epidermal extracts.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 19:02:39