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Titolo:
ISOLATION AND CHARACTERIZATION OF A BOVINE NEURAL-SPECIFIC PROTEIN (CRMP-2) CDNA HOMOLOGOUS TO UNC-33, A C-ELEGANS GENE IMPLICATED IN AXONAL OUTGROWTH AND GUIDANCE
Autore:
KAMATA T; SUBLESKI M; HARA Y; YUHKI N; KUNG HF; COPELAND NG; JENKINS NA; YOSHIMURA T; MODI W; COPELAND TD;
Indirizzi:
NCI,LAB BIOCHEM PHYSIOL,FCRDC,BLDG 567,RM 152 FREDERICK MD 21702 NHLBI,LAB BIOCHEM GENET,NIH BETHESDA MD 20892 NCI,VIRAL CARCINOGENESIS LAB,FCRDC FREDERICK MD 21702 NCI,SAIC FREDERICK,IRSP,FCRDC FREDERICK MD 21702 NCI,MAMMALIAN GENET LAB,ABL BASIC RES PROGRAM,FCRDC FREDERICK MD 21702 NCI,IMMUNOPATHOL LAB,FCRDC FREDERICK MD 21702
Titolo Testata:
Molecular brain research
fascicolo: 2, volume: 54, anno: 1998,
pagine: 219 - 236
SICI:
0169-328X(1998)54:2<219:IACOAB>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
LINKAGE MAP; FAMILY; IDENTIFICATION; LEUKEMIA; RECEPTOR; NEURONS; TOAD-64; KINASE; RAT;
Keywords:
NEURAL DEVELOPMENT; BOVINE CRMP-2; PROTEIN KINASE; UNC-33; CHROMOSOME MAPPING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
T. Kamata et al., "ISOLATION AND CHARACTERIZATION OF A BOVINE NEURAL-SPECIFIC PROTEIN (CRMP-2) CDNA HOMOLOGOUS TO UNC-33, A C-ELEGANS GENE IMPLICATED IN AXONAL OUTGROWTH AND GUIDANCE", Molecular brain research, 54(2), 1998, pp. 219-236

Abstract

We have cloned the cDNA encoding bovine CRMP-2 from bovine brains. A full length cDNA encoding bovine CRMP-2 was isolated and sequenced. The deduced amino acid sequence reveals that the gene encodes a protein of 572 amino acids and is highly homologous to Caenorhabditis elegans unc-33, which controls the guidance and outgrowth of neuronal axons. The CRMP-2 transcript was present in bovine brains but not non-neural tissues, and its protein product existed in both soluble and membrane-bound forms. The expression of CRMP-2 protein and mRNA was upregulated during neuronal differentiation of rat PC12 cells. Immunoprecipitationof PC12 cell extracts shows that CRMP-2 was co-immunoprecipitated with a 190 kDa protein (p190). Both CRMP-2 and p190 were phosphorylated on serine residues in vivo and in vitro in a kinase assay of CRMP-2 immune complexes. Immunocytochemistry shows that CRMP-2 was exclusively localized in both the central and peripheral nervous systems in mouse embryos and detectable in the adult brain although the level of CRMP-2 decreased. The protein was expressed in the axon, dendrite, and cytoplasm of postmitotic neurons and in the cytoplasm of oligodendrocytes and astrocytes. The CRMP-2 gene maps to the region of mouse chromosome 14 syntenic with human chromosome 8p21. Taken together, these data suggest multi-functional roles for CRMP-2 in developing and adult nervous systems, and the biological activity of CRMP-2 could be regulated by phosphorylation reaction. (C) 1998 Elsevier Science B.V.

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Documento generato il 22/01/20 alle ore 12:54:06