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Titolo:
SIMULTANEOUS DETERMINATION OF OXALATE, CITRATE AND SULFATE IN CHILDRENS PLASMA WITH ION CHROMATOGRAPHY
Autore:
HOPPE B; KEMPER MJ; HVIZD MG; SAILER DE; LANGMAN CB;
Indirizzi:
NORTHWESTERN UNIV,CHILDRENS MEM HOSP,DIV PEDIAT NEPHROL,2300 CHILDRENS PLAZA 37 CHICAGO IL 60614 UNIV CHILDRENS HOSP,DIV PEDIAT NEPHROL HAMBURG GERMANY DIONEX CHEM CORP WESTMONT IL 00000 NORTHWESTERN UNIV,CHILDRENS MEM HOSP,DIV PEDIAT NEPHROL CHICAGO IL 60614
Titolo Testata:
Kidney international
fascicolo: 5, volume: 53, anno: 1998,
pagine: 1348 - 1352
SICI:
0085-2538(1998)53:5<1348:SDOOCA>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHRONIC-RENAL-FAILURE; HEALTHY-SUBJECTS; URINARY OXALATE; GLYCOLATE EXCRETION; INORGANIC SULFATE; METABOLISM; HEMODIALYSIS; OXALOSIS; OXIDASE;
Keywords:
MINERAL METABOLISM; NEPHROLITHIASIS; PRIMARY HYPEROXALURIA; CHILDREN; NEPHROCALCINOSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
B. Hoppe et al., "SIMULTANEOUS DETERMINATION OF OXALATE, CITRATE AND SULFATE IN CHILDRENS PLASMA WITH ION CHROMATOGRAPHY", Kidney international, 53(5), 1998, pp. 1348-1352

Abstract

To improve our understanding of both diagnosis and treatment of diseases of oxalate metabolism, we first set out Lo establish a new ion-chromatographic method to determine normal plasma levels of oxalate, citrate and sulfate from single plasma samples. In 50 infants and children(23 girls, 27 boys, aged 0.2 to 17 years) with normal renal function,blood was drawn in Li-heparin tubes, placed on ice and preserved immediately with 40 mu l 1 M HCl/ml plasma in two ultracentrifugation steps. For measurement, plasma was injected onto an ion chromatography system with NaOH as the mobile phase. and then run as a linear gradient from 5 mM to 52.5 mM over 21 minutes. Analysis yielded measurable and reproducible oxalate (6.43 +/- 1.06 mu M/liter), citrate (79.3 +/- 27.4mu M/liter) and sulfate 235.0 +/- 85.3 mu M/liter) levels. without any age and gender specific differences. The least detectable plasma oxalate level was < 0.3 mu M with a high reliability and reproducibility (coefficient of variance 1.95 to 4.75%). In conclusion, we establisheda reproducible, precise method to determine the relevant plasma anions involved in mineral metabolism, which heretofore have not been easily measurable. Studies of diseases of oxalate and citrate metabolism are ongoing on the basis of the normal plasma values achieved this study.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 14:53:03