Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
IDENTIFICATION AND CHARACTERIZATION OF AN ESSENTIAL FAMILY OF INOSITOL POLYPHOSPHATE 5-PHOSPHATASES (INP51, INP52 AND INP53 GENE-PRODUCTS) IN THE YEAST SACCHAROMYCES-CEREVISIAE
Autore:
STOLZ LE; HUYNH CV; THORNER J; YORK JD;
Indirizzi:
DUKE UNIV,MED CTR,DEPT PHARMACOL & CANC BIOL,DUMC 3813 DURHAM NC 27710 DUKE UNIV,MED CTR,DEPT PHARMACOL & CANC BIOL DURHAM NC 27710 DUKE UNIV,MED CTR,DEPT BIOCHEM DURHAM NC 27710 UNIV CALIF BERKELEY,DEPT MOL & CELL BIOL,DIV BIOCHEM & MOL BIOL BERKELEY CA 94720
Titolo Testata:
Genetics
fascicolo: 4, volume: 148, anno: 1998,
pagine: 1715 - 1729
SICI:
0016-6731(1998)148:4<1715:IACOAE>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE 5-PHOSPHATASE; HUMAN-PLATELETS; 1,4,5-TRISPHOSPHATE 5-PHOSPHATASE; BIOCHEMICAL-CHARACTERIZATION; OSMOTIC STABILITY; 2ND MESSENGERS; PROTEIN-KINASE; CELL-CYCLE; DOMAINS; BRAIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
68
Recensione:
Indirizzi per estratti:
Citazione:
L.E. Stolz et al., "IDENTIFICATION AND CHARACTERIZATION OF AN ESSENTIAL FAMILY OF INOSITOL POLYPHOSPHATE 5-PHOSPHATASES (INP51, INP52 AND INP53 GENE-PRODUCTS) IN THE YEAST SACCHAROMYCES-CEREVISIAE", Genetics, 148(4), 1998, pp. 1715-1729

Abstract

We recently demonstrated that the S. cerevisiae INP51 locus (YIL002c)encodes an inositol polyphosphate 5-phosphatase. Here we describe tworelated yeast loci, INP52 (YNL106c) and INP53 (YOR109w). Like Inp51p,the primary structures of Inp52p and Inp53p resemble the mammalian synaptic vesicle-associated protein, synaptojanin, and contain a carboxy-terminal catalytic domain and an amino-terminal SAC1-like segment. Inp51p (108 kD), Inp52p (136 kD) and Inp53p (124 kD) are membrane-associated. Single null mutants (inp51, inp52, or inp53) are viable. Both inp51 inp52 and inp52 inp53 double mutants display compromised cell growth, whereas an inp51 inp53 double mutant does not. An inp51 inp52 inp53 triple mutant is inviable on standard medium, but call grow weakly on media supplemented with an osmotic stabilizer (1 M sorbitol). An inp51 mutation, and to a lesser degree an inp52 mutation, confers cold-resistant growth in a strain background that cannot grow at temperaturesbelow 15 degrees. Analysis of inositol metabolites in vivo showed measurable accumulation of phosphatidylinositol 4,5-bisphosphate in the inp51 mutant. Electron microscopy revealed plasma membrane invaginations and cell wall thickening in double mutants and the triple mutant grown in sorbitol-containing medium. A fluorescent dye that detects endocytic and vacuolar membranes suggests that the vacuole is highly fragmented in inp51 inp52 double mutants. Our observations indicate that Inp51p, Inp52p, and Inp53p have distinct functions and that substrates and/or products of inositol polyphosphate 5-phosphatases may have roles in vesicle trafficking, membrane structure, and/or cell wall formation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 09:43:33