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Titolo:
GENERATION OF ANTIBODIES TO HUMAN IL-12 AND AMPHIREGULIN BY IMMUNIZATION OF BALB C MICE WITH DIEPITOPE MULTIPLE ANTIGEN PEPTIDES/
Autore:
AHLBORG N; PAULIE S; BRAESCHANDERSEN S;
Indirizzi:
UNIV STOCKHOLM,DEPT IMMUNOL S-10691 STOCKHOLM SWEDEN
Titolo Testata:
Journal of immunological methods
fascicolo: 1, volume: 204, anno: 1997,
pagine: 23 - 32
SICI:
0022-1759(1997)204:1<23:GOATHI>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
T-CELL EPITOPES; CIRCUMSPOROZOITE PROTEIN; PLASMODIUM-FALCIPARUM; SYNTHETIC PEPTIDES; COVALENT LINKAGE; AMINO-ACIDS; IMMUNOGENICITY; SYSTEM; ANTIPEPTIDE; SPECIFICITY;
Keywords:
B-CELL EPITOPE; MONOCLONAL ANTIBODY; MULTIPLE ANTIGEN PEPTIDE; SPECIFIC ANTIBODIES; T-CELL HELP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
N. Ahlborg et al., "GENERATION OF ANTIBODIES TO HUMAN IL-12 AND AMPHIREGULIN BY IMMUNIZATION OF BALB C MICE WITH DIEPITOPE MULTIPLE ANTIGEN PEPTIDES/", Journal of immunological methods, 204(1), 1997, pp. 23-32

Abstract

Six peptide sequences derived from the human proteins/oligopeptides IL-12, amphiregulin and FALL-39 were synthesized in order to raise specific antibodies in Balb/c mice. Although peptides are valuable tools for generating specific antibodies, they are often poor immunogens due to their small size and lack of relevant T-cell epitopes. To circumvent these limitations, the human peptides were co-synthesized in diepitope multiple antigen peptides (MAP) with a known H-2(d)-restricted T helper-cell epitope. The importance of including a T-cell epitope in thediepitope MAPs was demonstrated by the fact that only one of the human peptides was immunogenic as a monoepitope MAP, lacking the T-cell epitope. Conversely, all diepitope MAPs generated potent antibody responses to the desired human peptides as well as to the T-cell epitope. A certain degree of variability of the antibody responses to the diepitope MAPs indicated that the alterable component, i.e. the human B-cell epitope, influenced the T-cell help elicited by the T-cell epitope. Still, the relative conformity of the B-cell responses suggests that this strategy is generally applicable for a rational production of specific antibodies. Moreover, antiserum to four diepitope MAPs recognized the corresponding full-length human protein/oligopeptides did monoclonal antibodies made against n-12- and amphiregulin-based MAPs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 07:46:29