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Titolo:
CONSTRUCTION AND CHARACTERIZATION OF AN NACL-SENSITIVE MUTANT OF HALOMONAS-ELONGATA IMPAIRED IN ECTOINE BIOSYNTHESIS
Autore:
GOLLER K; OFER A; GALINSKI EA;
Indirizzi:
UNIV MUNSTER,INST BIOCHEM,WILHELM KLEMM STR 2 D-48149 MUNSTER GERMANY UNIV MUNSTER,INST BIOCHEM D-48149 MUNSTER GERMANY UNIV BONN,INST MIKROBIOL & BIOTECHNOL D-53115 BONN GERMANY
Titolo Testata:
FEMS microbiology letters
fascicolo: 2, volume: 161, anno: 1998,
pagine: 293 - 300
SICI:
0378-1097(1998)161:2<293:CACOAN>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMPATIBLE SOLUTES; TRANSPOSON MUTAGENESIS; HALOPHILIC EUBACTERIA; ESCHERICHIA-COLI; TETRAHYDROPYRIMIDINES; STRESS; DERIVATIVES; OSMOLYTES; TREHALOSE; BACTERIA;
Keywords:
HALOMONAS ELONGATA; COMPATIBLE SOLUTE; ECTOINE BIOSYNTHESIS; TRANSPOSON MUTAGENESIS; SALT-SENSITIVE MUTANT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
K. Goller et al., "CONSTRUCTION AND CHARACTERIZATION OF AN NACL-SENSITIVE MUTANT OF HALOMONAS-ELONGATA IMPAIRED IN ECTOINE BIOSYNTHESIS", FEMS microbiology letters, 161(2), 1998, pp. 293-300

Abstract

Using transposon mutagenesis we generated a salt-sensitive mutant of the halophilic eubacterium Halomonas elongata impaired in the biosynthesis of the compatible solute ectoine. HPLC determinations of the cytoplasmic solute content showed the accumulation of a biosynthetic precursor of ectoine, L-2,4-diaminobutyric acid. Ectoine and hydroxyectoinewere not detectable. This mutant failed to grow in minimal medium with NaCl concentrations exceeding 4%. However, when supplemented with organic osmolytes, the ability to grow in high-salinity medium (15% and higher) was regained. We cloned and sequenced the regions flanking thetransposon insertion in the N. elongata chromosome. Sequence comparisons with known proteins revealed significant similarity of the mutatedgene to the L-2,4-diaminobutyric acid acetyltransferase from the ectoine biosynthetic pathway in Marinococcus halophilus. Analysis of a PCRproduct demonstrated that the ectoine biosynthetic genes (ectABC) follow the same order as in M. halophilus. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 02:11:22