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Titolo:
PRINCIPLES AND CHARACTERISTICS OF MULTICOLOR FLUORESCENCE IMAGING OF PLANTS
Autore:
BUSCHMANN C; LICHTENTHALER HK;
Indirizzi:
UNIV KARLSRUHE,BOT INST 2,KAISERSTR 12 D-76128 KARLSRUHE GERMANY
Titolo Testata:
Journal of plant physiology
fascicolo: 2-3, volume: 152, anno: 1998,
pagine: 297 - 314
SICI:
0176-1617(1998)152:2-3<297:PACOMF>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHLOROPHYLL-A-FLUORESCENCE; LASER-INDUCED FLUORESCENCE; PRIMARY-CELL WALL; FERULIC ACID; PHENOLIC COMPONENTS; INDUCTION KINETICS; GLECHOMA-HEDERACEA; CO2 ASSIMILATION; RATIO F690/F730; BARLEY LEAVES;
Keywords:
CHLOROPHYLL FLUORESCENCE; BLUE-GREEN FLUORESCENCE; FLUORESCENCE IMAGES; FLUORESCENCE RATIOS; STRESS DETECTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
74
Recensione:
Indirizzi per estratti:
Citazione:
C. Buschmann e H.K. Lichtenthaler, "PRINCIPLES AND CHARACTERISTICS OF MULTICOLOR FLUORESCENCE IMAGING OF PLANTS", Journal of plant physiology, 152(2-3), 1998, pp. 297-314

Abstract

Green plants illuminated with UV-radiation emit a blue and green fluorescence as well as a red and far-red chlorophyll fluorescence with maxima near 440, 520, 690 and 740 nm, respectively. In contrast to pointdata measurements, the establishment of a high spatial resolution multi-colour fluorescence imaging system, the Karlsruhe/Strasbourg Fluorescence Imaging System (FIS), permits to simultaneously screen several hundred picture elements (pixels) of the leaf in the four fluorescencebands of plants, and opens new possibilities for physiology measurements and early stress detection. This review describes the principles and particular characteristics a) of the plants' fluorescence signatures, b) of fluorescence imaging in general and c) of the Karlsruhe/Strasbourg high resolution multi-colour fluorescence imaging system FIS. The origin of the fluorescence signals in the blue and green (cinnamic acids and other plant phenolics), the red and far-red (chlorophyll alpha) and the factors influencing these fluorescence signatures are explained. By means of an intensified video camera, fluorescence images with several hundred picture elements are taken, not only of the red and far-red Chi fluorescence, but also of the blue and green plant fluorescence, as multicolour images. Via computer-aided data processing one obtains false colour images not only of the fluorescence intensity, butalso of the fluorescence ratios of whole leaves. The fluorescence ratios blue/red (F440/F630) and blue/far-red (F440/F740) proved to be very sensitive early stress and strain indicators of plants. The fluorescence ratio F690/F740 is an indicator of the in situ Chi content. By fluorescence imaging of the variable Chi fluorescence (Rfd-values) one can also simultaneously measure the potential photosynthetic activity of all points of a leaf. At short-term stress, the fluorescence ratio blue/green (F440/F520) is relatively stable, but changes at long-term stress, often by an increase of the green fluorescence emission. Due tothe high statistic confidence (large pixel numbers) and the excellentpossibilities to detect specific distribution gradients and local irregularities in fluorescence emission and fluorescence ratios over the leaf area, fluorescence imaging is a superior means for early stress and damage detection in plants.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 06:13:24