Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
DETECTION AND ENUMERATION OF A GENETICALLY-MODIFIED FUNGUS IN SOIL ENVIRONMENTS BY QUANTITATIVE COMPETITIVE POLYMERASE-CHAIN-REACTION
Autore:
BAEK JM; KENERLEY CM;
Indirizzi:
TEXAS A&M UNIV,DEPT PLANT PATHOL & MICROBIOL COLLEGE STN TX 77843 TEXAS A&M UNIV,DEPT PLANT PATHOL & MICROBIOL COLLEGE STN TX 77843
Titolo Testata:
FEMS microbiology, ecology
fascicolo: 4, volume: 25, anno: 1998,
pagine: 419 - 428
SICI:
0168-6496(1998)25:4<419:DAEOAG>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIRECT DNA EXTRACTION; GLIOCLADIUM-VIRENS; BACTERIAL-DNA; MICROBIAL DNA; RAPID METHOD; LOW NUMBERS; SEDIMENTS; TRICHODERMA; MICROORGANISMS; BIOCONTROL;
Keywords:
COMPETITIVE POLYMERASE CHAIN REACTION; GENETICALLY ENGINEERED MICROORGANISM; TRICHODERMA VIRENS; DETECTION; SOIL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
J.M. Baek e C.M. Kenerley, "DETECTION AND ENUMERATION OF A GENETICALLY-MODIFIED FUNGUS IN SOIL ENVIRONMENTS BY QUANTITATIVE COMPETITIVE POLYMERASE-CHAIN-REACTION", FEMS microbiology, ecology, 25(4), 1998, pp. 419-428

Abstract

A procedure of quantitative competitive polymerase chain reaction wasdeveloped for detection and quantitation of a genetically modified strain of Trichoderma virens (GvT6) in the soil environment. A 1.0-kb coding region of the opd (organophosphate degrading) gene on GvT6 genomic DNA tc as co-amplified with a standard DNA. The standard DNA was a 1.5-kb fragment of the gpd (glyceraldehyde-3-phosphate dehydrogenase) gene of T. virens containing opd-primer-binding sites on the ends. Soilsamples containing various types of propagules of GvT6 were treated with a combined procedure of SDS-heating and bead-beating and the released nucleic acids were extracted and purified. Relative efficiency of T., virens DNA recovery from soil samples was about 85-95% compared with DNA yield from spore suspensions. Following the PCR, the product ratio between the target and the standard was plotted against a dilutionseries of the standard which was added into the amplification reaction. Then, the concentration of the opd gene and the GvT6 in the soil sample were estimated. Despite the differences in size and nucleotide sequences between the target and the standard? they were amplified with similar efficiency. The detection limit of the PCR was 10-1000 times lower compared to traditional dilution plating. The quantitative competitive PCR technique will enhance our ability to understand the ecologyof soilborne fungi in the soil environment. (C) 1998 Federation of European Microbiological Societies. Published bq Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 01:05:00