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Titolo: GLUCOCORTICOID REGULATION OF CORTICOTROPIN-RELEASING FACTOR(1) RECEPTOR EXPRESSION IN PITUITARY-DERIVED ATT-20 CELLS
Autore: IREDALE PA; DUMAN RS;
- Indirizzi:
- YALE UNIV,SCH MED,CONNECTICUT MENTAL HLTH CTR,DEPT PSYCHIAT,LAB MOL PSYCHIAT,34 PK ST NEW HAVEN CT 06508 YALE UNIV,SCH MED,CONNECTICUT MENTAL HLTH CTR,DEPT PSYCHIAT,LAB MOL PSYCHIAT NEW HAVEN CT 06508 YALE UNIV,SCH MED,CONNECTICUT MENTAL HLTH CTR,DEPT PHARMACOL,LAB MOL PSYCHIAT NEW HAVEN CT 06508
- Titolo Testata:
- Molecular pharmacology
fascicolo: 5,
volume: 51,
anno: 1997,
pagine: 794 - 799
- SICI:
- 0026-895X(1997)51:5<794:GROCFR>2.0.ZU;2-1
- Fonte:
- ISI
- Lingua:
- ENG
- Soggetto:
- SMOOTH-MUSCLE CELLS; OPIOMELANOCORTIN GENE-TRANSCRIPTION; AGONIST-INDUCED DESTABILIZATION; FACTOR CRF RECEPTORS; MESSENGER-RNA LEVELS; C-JUN; DOWN-REGULATION; ACTH-SECRETION; UP-REGULATION; BINDING;
- Tipo documento:
- Article
- Natura:
- Periodico
- Settore Disciplinare:
- Science Citation Index Expanded
- Science Citation Index Expanded
- Citazioni:
- 40
- Recensione:
- Indirizzi per estratti:
-
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- Citazione:
- P.A. Iredale e R.S. Duman, "GLUCOCORTICOID REGULATION OF CORTICOTROPIN-RELEASING FACTOR(1) RECEPTOR EXPRESSION IN PITUITARY-DERIVED ATT-20 CELLS", Molecular pharmacology, 51(5), 1997, pp. 794-799
Abstract
Corticotropin-releasing factor (CRF) receptors represent one of the primary sites for negative feedback of the pituitary by adrenocortical glucocorticoid hormones; however, the molecular mechanisms involved have yet to be elucidated. The present study examines the mechanisms by which glucocorticoids regulate CRF-RI expression in the pituitary cellline, AtT-20. Treatment of these cells with dexamethasone resulted ina concentration- and time-dependent inhibition of CRF-RI mRNA that was significant by 1 hr and maximal after 4 hr. Levels of CRF-R1 mRNA then returned to control levels after 24 hr. Similar changes were observed when the cells were treated with corticosterone. Pro-opiomelanocortin mRNA was also decreased after dexamethasone pretreatment; however, the time course was much slower with a significant effect only detected after 6 hr. Further analysis of the mechanisms that mediate glucocorticoid regulation of CRF-R1 mRNA was conducted. These studies demonstrated that glucocorticoid incubation significantly decreases the rate of CRF-R1 gene transcription, as determined by nuclear run-on analysis. In addition, the results demonstrate that glucocorticoid incubation significantly decreases CRF-RI mRNA stability by approximately 50%. Thedown-regulation of CRF-R1 mRNA was dependent on de novo protein synthesis, as it was blocked by pretreatment with cycloheximide. This represents a novel mechanism for glucocorticoid negative feedback regulation of CRF-R1 expression.
ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 00:33:27