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Titolo:
EFFECT OF POSTMORTEM STORAGE ON THE Z-LINE REGION OF TITIN IN BOVINE MUSCLE
Autore:
BOYERBERRI C; GREASER ML;
Indirizzi:
UNIV WISCONSIN,MUSCLE BIOL LAB MADISON WI 53706 UNIV WISCONSIN,MUSCLE BIOL LAB MADISON WI 53706
Titolo Testata:
Journal of animal science
fascicolo: 4, volume: 76, anno: 1998,
pagine: 1034 - 1044
SICI:
0021-8812(1998)76:4<1034:EOPSOT>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONGISSIMUS MUSCLE; ELASTIC PROPERTIES; STRIATED-MUSCLE; MONOCLONAL-ANTIBODIES; 1,200-KDA SUBFRAGMENT; CONNECTIN FILAMENTS; ALPHA-CONNECTIN; SKELETAL-MUSCLE; F-ACTIN; DEGRADATION;
Keywords:
POSTMORTEM CHANGES; MYOFIBRILS; BOVIDAE; DEGRADATION; MUSCLES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
C. Boyerberri e M.L. Greaser, "EFFECT OF POSTMORTEM STORAGE ON THE Z-LINE REGION OF TITIN IN BOVINE MUSCLE", Journal of animal science, 76(4), 1998, pp. 1034-1044

Abstract

Myofibrils were prepared from bovine muscles (cutaneous trunci, rectus abdominis, psoas major, and masseter) and compared between differentaging periods at 4 degrees C (0, 1, 2, 4, 8, and 16 d). Myofibrils were stained with an antibody directed against a 56-kDa fragment (FE-RE)of titin located in the Z-line region. Unaged myofibrils from all four muscles showed a single stained band at the Z-line with similar intensities. Postmortem time did not significantly affect the total amountof fluorescence in the sarcomere, suggesting the titin FE-RE epitope was not degraded nor were titin fragments containing this epitope released during storage. However, the fluorescence patterns were altered. The relative fluorescence intensity at the Z-line decreased but that in the I-band increased gradually, showing the translocation of some titin FE-RE epitopes during the aging period. This suggested that a cleavage occurred in a region of titin very close to the Z-line during postmortem storage. Usually the position of maximum fluorescence remainedat the Z-line, although about 1/3 of the myofibrils from rectus abdominis showed a two-band pattern around the Z-line after 16 d of aging. The titin changes observed may be related to the increased fragility of the myofibril and the improvement of meat tenderness during postmortem storage.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/08/20 alle ore 16:42:27