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Titolo:
PROMOTER STRUCTURES AND DIFFERENTIAL RESPONSES TO VIRAL AND NONVIRAL INDUCERS OF CHICKEN TYPE-I INTERFERON GENES
Autore:
SICK C; SCHULTZ U; MUNSTER U; MEIER J; KASPERS B; STAEHELI P;
Indirizzi:
UNIV FREIBURG,INST MED MIKROBIOL & HYG,DEPT VIROL,ABT VIROL,HERMANN HERDER STR 11 D-79008 FREIBURG GERMANY UNIV FREIBURG,INST MED MIKROBIOL & HYG,DEPT VIROL,ABT VIROL D-79008 FREIBURG GERMANY UNIV MUNICH,INST TIERPHYSIOL D-80539 MUNICH GERMANY
Titolo Testata:
The Journal of biological chemistry
fascicolo: 16, volume: 273, anno: 1998,
pagine: 9749 - 9754
SICI:
0021-9258(1998)273:16<9749:PSADRT>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALPHA PROMOTER; INDUCTION; VIRUS; EXPRESSION; CELLS; GAMMA; INDUCIBILITY; TROPHOBLAST; IMIQUIMOD; ELEMENTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
C. Sick et al., "PROMOTER STRUCTURES AND DIFFERENTIAL RESPONSES TO VIRAL AND NONVIRAL INDUCERS OF CHICKEN TYPE-I INTERFERON GENES", The Journal of biological chemistry, 273(16), 1998, pp. 9749-9754

Abstract

Two serologically distinct type I interferons (IFNs), designated ChIFN1 and ChIFN2, are known in the chicken. ChIFN1 is encoded by a familyof 10 or more genes, whereas ChIFN2 is encoded by a single gene. We show here that ChIFN1 and ChIFN2 transcripts are both strongly induced by Newcastle disease virus in primary chicken macrophages, By contrast, oral administration of the imidazoquinoline S-28463, which selectively induces IFN-alpha in mammals, led to a rapid accumulation of ChIFN1(but not ChIFN2) transcripts in adult chicken spleen and thymus. The 5'-upstream region of the ChIFN2 gene contains a NF-kappa B consensus motif flanked by a sequence element that could serve as a binding sitefor transcription factor IRF-1, reminiscent of mammalian IFN-beta promoters, and it mediated powerful virus inducibility in a duck fibroblast cell line when cloned in front of a promoterless luciferase reporter gene. The 5'-upstream region of the cloned ChIFN1 gene contains two putative binding sites for IRF-1, but lacks NF-kappa B-binding sites, and it did not respond well to virus in transfected cells, Thus, the promoters of ChIFN1 and ChIFN2 genes not only exhibited differential responses to nonviral inducers in vivo, but also differed in structure and response to virus in transfected cells. These findings indicate that ChIFN2 represents the avian homolog of mammalian IFN-beta, whereas ChIFN1 seems to correspond to mammalian IFN-alpha.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 13:04:45