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Titolo:
CLONING AND IN-SITU HYBRIDIZATION ANALYSIS OF ESTROGEN-RECEPTOR IN THE DEVELOPING GONAD OF THE RED-EARED SLIDER TURTLE, A SPECIES WITH TEMPERATURE-DEPENDENT SEX DETERMINATION
Autore:
BERGERON JM; GAHR M; HORAN K; WIBBELS T; CREWS D;
Indirizzi:
UNIV TEXAS,DEPT ZOOL,INST REPROD BIOL AUSTIN TX 78712 MAX PLANCK INST VERHALTENSPHYSIOL D-8130 STARNBERG GERMANY UNIV CHICAGO HOSP,DEPT OBSTET & GYNECOL CHICAGO IL 60637 UNIV ALABAMA,DEPT BIOL BIRMINGHAM AL 35294
Titolo Testata:
Development, growth & differentiation
fascicolo: 2, volume: 40, anno: 1998,
pagine: 243 - 254
SICI:
0012-1592(1998)40:2<243:CAIHAO>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER RIBONUCLEIC-ACIDS; TRACHEMYS-SCRIPTA; SEQUENCE HOMOLOGY; EXPRESSION; AROMATASE; IDENTIFICATION; CDNA;
Keywords:
ESTROGEN; ESTROGEN RECEPTOR; GONADS; TEMPERATURE-DEPENDENT SEX DETERMINATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
J.M. Bergeron et al., "CLONING AND IN-SITU HYBRIDIZATION ANALYSIS OF ESTROGEN-RECEPTOR IN THE DEVELOPING GONAD OF THE RED-EARED SLIDER TURTLE, A SPECIES WITH TEMPERATURE-DEPENDENT SEX DETERMINATION", Development, growth & differentiation, 40(2), 1998, pp. 243-254

Abstract

Many reptiles exhibit temperature-dependent sex determination where the incubation temperature of the egg determines the gonadal sex of theindividual. If exogenous estrogen is administered during the temperature-sensitive period to embryos incubating at a male-producing temperature, the temperature effects can be overridden and females will be produced. Inhibiting production of endogenous estrogens at female-biasedincubation temperatures results in embryos developing as males ratherthan females. Thus, estrogen-estrogen receptor-dependent mechanisms appear to play a key role in female sex determination. The present study characterized the expression of the estrogen receptor during the critical period of temperature sensitivity in the red-eared slider turtle, Trachemys scripta. Polymerase chain reaction was used to amplify estrogen receptor cDNA. A portion of the estrogen receptor cDNA was used to produce probes for in situ hybridization analyses' to localize and quantitate levels of estrogen receptor mRNA at different stages of development in embryos from different incubation temperatures. Estrogen receptor mRNA is expressed in the gonadal tissues of both putative males and putative females even before the gonads begin to resolve as ovaries or testes. There is a greater abundance of estrogen receptor mRNA in putative females at the beginning of the temperature-sensitive period as compared to putative males. In embryos from a female-producing incubation temperature. levels of estrogen receptor mRNA are higher in the beginning of the temperature-sensitive window compared to levels after the ovary is differentiated. These results support the hypothesisthat estrogen-estrogen receptor dependent processes are important during sex determination and gonadal differentiation in temperature-dependent sex determination.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 06:50:19