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Titolo:
GOLGI VESICULATION AND LYSOSOME DISPERSION IN CELLS LACKING CYTOPLASMIC DYNEIN
Autore:
HARADA A; TAKEI Y; KANAI Y; TANAKA Y; NONAKA S; HIROKAWA N;
Indirizzi:
UNIV TOKYO,GRAD SCH MED,DEPT ANAT & CELL BIOL,BUNKYO KU TOKYO 113 JAPAN UNIV TOKYO,GRAD SCH MED,DEPT ANAT & CELL BIOL,BUNKYO KU TOKYO 113 JAPAN
Titolo Testata:
The Journal of cell biology
fascicolo: 1, volume: 141, anno: 1998,
pagine: 51 - 59
SICI:
0021-9525(1998)141:1<51:GVALDI>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIN-RELATED PROTEIN; MEMBRANOUS ORGANELLES; RETROGRADE TRANSPORT; AXONAL-TRANSPORT; MITOTIC SPINDLES; MOTOR ENZYME; RAT-BRAIN; MICROTUBULES; LOCALIZATION; COMPLEX;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
A. Harada et al., "GOLGI VESICULATION AND LYSOSOME DISPERSION IN CELLS LACKING CYTOPLASMIC DYNEIN", The Journal of cell biology, 141(1), 1998, pp. 51-59

Abstract

Cytoplasmic dynein, a minus end-directed, microtubule-based motor protein, is thought to drive the movement of membranous organelles and chromosomes. It is a massive complex that consists of multiple polypeptides. Among these polypeptides, the cytoplasmic dynein heavy chain (cDHC) constitutes the major part of this complex. To elucidate the function of cytoplasmic dynein, we have produced mice lacking cDHC by gene targeting. cDHC(-/-) embryos were indistinguishable from cDHC(+/-) or cDHC(+/+) littermates at the blastocyst stage. However, no cDHC(-/-) embryos were found at 8.5 d postcoitum. When cDHC(-/-) blastocysts were cultured in vitro, they showed interesting phenotypes, First, the Golgi complex became highly vesiculated and distributed throughout the cytoplasm. Second, endosomes and lysosomes were not concentrated near thenucleus but were distributed evenly throughout the cytoplasm. Interestingly, the Golgi ''fragments'' and lysosomes were still found to be attached to microtubules. These results show that cDHC is essential forthe formation and positioning of the Golgi complex, Moreover, cDHC isrequired for cell proliferation and proper distribution of endosomes and lysosomes, However, molecules other than cDHC might mediate attachment of the Golgi complex and endosomes/lysosomes to microtubules.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 22:21:15