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Titolo:
NEUROSTEROID MODULATION OF GABA BINDING-SITES IN DEVELOPING AVIAN CENTRAL-NERVOUS-SYSTEM
Autore:
VIAPIANO MS; DENOVARA AMM; DEPLAZAS SF;
Indirizzi:
UNIV BUENOS AIRES,FAC MED,INST BIOL CELULAR & NEUROCIENCIAS,PARAGUAY 2155 3P RA-1121 BUENOS AIRES DF ARGENTINA UNIV BUENOS AIRES,FAC MED,INST BIOL CELULAR & NEUROCIENCIAS RA-1121 BUENOS AIRES DF ARGENTINA
Titolo Testata:
Neurochemistry international
fascicolo: 3, volume: 32, anno: 1998,
pagine: 291 - 298
SICI:
0197-0186(1998)32:3<291:NMOGBI>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHICK OPTIC LOBE; ACID RECEPTOR-BINDING; RAT-BRAIN; POSTNATAL-DEVELOPMENT; BENZODIAZEPINE RECEPTOR; STEROID MODULATION; A RECEPTORS; AFFINITY; COMPLEX; PROGESTERONE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
M.S. Viapiano et al., "NEUROSTEROID MODULATION OF GABA BINDING-SITES IN DEVELOPING AVIAN CENTRAL-NERVOUS-SYSTEM", Neurochemistry international, 32(3), 1998, pp. 291-298

Abstract

Our aim was to examine the effect of the potent neurosteroid 3 alpha-hydroxy-5 alpha-pregnan-20-one (3 alpha,5 alpha-P) on [H-3]-GABA binding to its receptor sites in the chick optic lobe. Binding was performed on synaptic membranes isolated at different stages of development and two different membrane preparation procedures were applied to exposehigh and low affinity GABA binding sites. The addition of 3 alpha,5 alpha-P was shown to increase [H-3]-GABA binding in an age-and concentration-dependent manner. Maximal stimulation for low affinity GABA binding sites was observed at hatching (130% enhancement), in fresh-washedas well as in frozen membranes. Saturation analysis performed on bothmembrane types disclosed that 3 alpha,5 alpha-P increases the affinity of low affinity GABA binding sites without altering their maximal binding capacity. On the other hand, the augmenting effect at high affinity sites, displayed only in frozen membranes, was roughly 50% for alldevelopmental stages. However, their saturation binding parameters remained unaltered in the presence of the steroid, suggesting that stimulation of such sites seems due to interference exerted by the low affinity site population. Findings indicate that 3 alpha,5 alpha-P acts asan allosteric modulator only for low affinity GABA binding sites, displaying an age-dependent profile probably related to plastic events during visual pathway development. (C) 1998 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 00:03:32