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Titolo:
MODERATE DISCRIMINATION OF REP-1 BETWEEN RAB7-GDP AND RAB7-GTP ARISESFROM A DIFFERENCE OF AN ORDER OF MAGNITUDE IN DISSOCIATION RATES
Autore:
ALEXANDROV K; SIMON I; IAKOVENKO A; HOLZ B; GOODY RS; SCHEIDIG AJ;
Indirizzi:
MAX PLANCK INST MOL PHYSIOL,DEPT PHYS BIOCHEM,RHEINLANDDAMM 201 D-44139 DORTMUND GERMANY MAX PLANCK INST MOL PHYSIOL,DEPT PHYS BIOCHEM D-44139 DORTMUND GERMANY
Titolo Testata:
FEBS letters
fascicolo: 3, volume: 425, anno: 1998,
pagine: 460 - 464
SICI:
0014-5793(1998)425:3<460:MDORBR>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAB ESCORT PROTEIN; GTP-BINDING PROTEINS; N-TERMINAL DOMAIN; GERANYLGERANYL TRANSFERASE; MEMBRANE-FUSION; COMPONENT-A; INHIBITOR; GDI; ENDOCYTOSIS; ASSOCIATION;
Keywords:
RAB7; REP-1; FLUORESCENCE SPECTROSCOPY; KINETICS; SMALL GTP BINDING PROTEIN; VESICULAR TRANSPORT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
K. Alexandrov et al., "MODERATE DISCRIMINATION OF REP-1 BETWEEN RAB7-GDP AND RAB7-GTP ARISESFROM A DIFFERENCE OF AN ORDER OF MAGNITUDE IN DISSOCIATION RATES", FEBS letters, 425(3), 1998, pp. 460-464

Abstract

The kinetics of the interaction of Rab7 with REP-1 have been investigated using the fluorescence of GDP and GTP analogs at the active site of Rab7. The results show that REP-I has higher affinity for the GDP bound form of Rab7 (K-d = 1 nM) than for the GTP bound form (K-d = 20 nM). Both affinities should still be sufficient for the formation of stable completes in the cell. The association reaction proceeds in two steps for the GDP bound form. The initial step is fast (k(+1)=ca, 10(7)M-1 s(-1)) and concentration dependent while the second represents a slow equilibration (k(+2)+k(-2)=3.5 s(-1)) which has little effect on the overall equilibrium. The difference in affinity of the two nucleotide bound forms arises from a difference in dissociation rates (0.012 s(-1) for Rab7 GDP and 0.2 s(-1) for Rab7.GTP). (C) 1998 Federation ofEuropean Biochemical Societies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 11:04:02