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Titolo:
THE ANTIOXIDATIVE EFFECTS OF THE ISOFLAVAN GLABRIDIN ON ENDOGENOUS CONSTITUENTS OF LDL DURING ITS OXIDATION
Autore:
BELINKY PA; AVIRAM M; FUHRMAN B; ROSENBLAT M; VAYA J;
Indirizzi:
MIGAL GALILEE TECHNOL CTR,POB 90000 IL-12100 ROSH PINNA ISRAEL MIGAL GALILEE TECHNOL CTR IL-12100 ROSH PINNA ISRAEL MIGAL GALILEE TECHNOL CTR,LAB NAT CPDS MED USE IL-10200 KIRYAT SHMONAISRAEL TECHNION ISRAEL INST TECHNOL,BRUCE RAPPAPORT FAC MED,RAMBAM MED CTR,LIPID RES LAB IL-31096 HAIFA ISRAEL
Titolo Testata:
Atherosclerosis
fascicolo: 1, volume: 137, anno: 1998,
pagine: 49 - 61
SICI:
0021-9150(1998)137:1<49:TAEOTI>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; HUMAN MONOCYTE-MACROPHAGES; VITAMIN-E; LIPID-PEROXIDATION; ALPHA-TOCOPHEROL; GAS-CHROMATOGRAPHY; INVITRO OXIDATION; ENDOTHELIAL-CELLS; OXIDIZED LIPIDS; BETA-CAROTENE;
Keywords:
GLABRIDIN; LICORICE; ISOFLAVAN; LDL OXIDATION; CHOLESTERYL LINOLEATE HYDROPEROXIDE; OXYSTEROLS; VITAMIN E; CAROTENOIDS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
76
Recensione:
Indirizzi per estratti:
Citazione:
P.A. Belinky et al., "THE ANTIOXIDATIVE EFFECTS OF THE ISOFLAVAN GLABRIDIN ON ENDOGENOUS CONSTITUENTS OF LDL DURING ITS OXIDATION", Atherosclerosis, 137(1), 1998, pp. 49-61

Abstract

The effect of the consumption of glabridin, an isoflavan isolated from Glycyrrhiza glabra (licorice) root, on the susceptibility of low density lipoprotein (LDL) to oxidation was studied in atherosclerotic apolipoprotein E deficient (E degrees mice) and was compared with that ofthe known flavonoids, quercetin and catechin. Glabridin inhibitory activity on in vitro oxidation of human LDL was also investigated by determining the formation of lipid peroxides and oxysterols and the consumption of LDL-associated lipophilic antioxidants. Determination of theextent of LDL oxidation by measuring the formation of thiobabituric acid reactive substances (TBARS) after 2 h of LDL incubation with CuSO4(10 mu M) or 2,2'-azobis (2-amidino-propane) dihydrochloride (AAPH) (5 mM), revealed that glabridin or quercetin consumption resulted in a 53 and 54% reduction in copper ion induced oxidation, respectively, and a 95 and 83% reduction in AAPH induced LDL oxidation, respectively. No inhibition was obtained with consumption of catechin. About 80% of glabridin was found to bind to the LDL human particle. In the in vitrooxidation of LDL induced by AAPH (5 mM), glabridin inhibited the formation of TBARS, lipid peroxides and cholesteryl linoleate hydroperoxide (CLOOH) at all the concentrations tested (5-60 mu M), while in oxidation induced by copper ions (10 mu M), glabridin exhibited a pro-oxidant activity at concentrations lower than 20 mu M, and a clear antioxidant activity at concentrations greater than 20 mu M. Glabridin (30 mu M) inhibited the formation of cholest-5-ene-3,7-diol (7-hydroxycholesterol): cholest-5-ene-3-ol-7-one (7-ketocholesterol) and cholestan-5,6-epoxy-3-ol (5,6-epoxycholesterol) after 6 h of AAPH induced LDL oxidation, by 55, 80 and 40%, respectively, and after 6 h of copper ion induced LDL oxidation, by 73, 94 and 52%, respectively. Glabridin also inhibited the consumption of beta-carotene and lycopene by 38 and 52%, respectively, after 0.5 h of LDL oxidation with AAPH, but failed to protect vitamin E. The in vivo and in vitro reduction of the susceptibility of LDL to oxidation obtained with glabridin, may be related to the absorption or binding of glabridin to the LDL particle and subsequent protection of LDL from oxidation by inhibiting the formation of lipid peroxides and oxysterols, and by protecting LDL associated carotenoids. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.

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Documento generato il 18/02/20 alle ore 14:35:17