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Titolo:
TRYPANOSOMA-CRUZI - IMMUNE-RESPONSE AND FUNCTIONAL HEART DAMAGE-INDUCED IN MICE BY THE MAIN LINEAR B-CELL EPITOPE OF PARASITE RIBOSOMAL P-PROTEIN
Autore:
MOTRAN CC; CERBAN FM; RIVAROLA W; IOSA D; DECIMA EV;
Indirizzi:
UNIV NACL CORDOBA,FAC CIENCIAS QUIM,DEPT BIOQUIM CLIN,CC61 AG POSTAL 4 RA-5000 CORDOBA ARGENTINA UNIV NACL CORDOBA,FAC MED,CATEDRA FIS BIOMED RA-5000 CORDOBA ARGENTINA CTR PRIVADO MED CORDOBA ARGENTINA
Titolo Testata:
Experimental parasitology
fascicolo: 3, volume: 88, anno: 1998,
pagine: 223 - 230
SICI:
0014-4894(1998)88:3<223:T-IAFH>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHRONIC CHAGAS-DISEASE; SYSTEMIC LUPUS-ERYTHEMATOSUS; HUMORAL AUTOIMMUNE-RESPONSE; AUTOANTIBODY PRODUCTION; NUCLEOTIDE-SEQUENCE; TRANSGENIC MICE; SELF-TOLERANCE; ANTIGEN; MOUSE; ANTIBODY;
Keywords:
TRYPANOSOMA CRUZI; PROTOZOA; CHAGAS DISEASE; PARASITE; B-CELL EPITOPE; MOLECULAR MIMICRY; PROTECTION; PATHOGENESIS; RIBOSOMAL P PROTEINS; ANTIBODY, SYNTHETIC PEPTIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
C.C. Motran et al., "TRYPANOSOMA-CRUZI - IMMUNE-RESPONSE AND FUNCTIONAL HEART DAMAGE-INDUCED IN MICE BY THE MAIN LINEAR B-CELL EPITOPE OF PARASITE RIBOSOMAL P-PROTEIN", Experimental parasitology, 88(3), 1998, pp. 223-230

Abstract

We report herein on the specific and autoimmune humoral response generated by the immunization of mice with the R13 synthetic peptide coupled to a carrier protein, OVA. This peptide correspons to the C-terminal region of Trypanosoma cruzi ribosomal P1 and P2 proteins (EEEDDDMGFGLFD), a sequence that differs from the other eukariotic P consensus sequence (EESDDDMGFGLFD) only in a nonconservative amino acid substitution. The antibody response studied by ELISA revealed that all R13-immunized mice had antibodies against R13, consisting mainly of IgG1 and IgG2 isotypes, even though IgG3 and IgE isotypes were also observed. Theself-reactivity of anti-R13 sera assayed by immunoblot, revealed thatall sera contained IgG antibodies binding to mouse and human 38-kDa heart antigen. This antigenic band binds several immunoglobulin isotypes (IgG2 > IgG3 > IgE > IgG1). The specificity of anti-R13 antibodies analyzed by competitive inhibition of R13 ELISA using R13 and R7 (MGFGLFD) peptides revealed that the reactivity of the induced anti-P antibodies was not absorbed by R7. Therefore, the main immunogenic region ofR13 for mouse would be EEEDDD, which contains the amino acid substitution. In parallel with this humoral response, both partial protection and heart damage were observed in R13-immunized mice. In fact, the R13-immunized mice showed significantly lower parasitemia and longer survival than the control animals. In addition, all R13-immunized mice showed electrocardiographic changes (bradycardia, prolonged PQ segment, and intraventricular conduction disturbances), which are typical findings in Chagas disease patients. This study represents the first definitive report in which one defined B-cell epitope, the single peptide R13from T. cruzi, coupled to a carrier protein was able to induce specific and autoreactive antibodies as well as to generate heart functionalalterations. (C) 1998 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:27:01