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Titolo:
PURIFICATION AND CHARACTERIZATION OF GLYCOPROTEIN FROM THE SKIN MUCUSOF THE RAINBOW-TROUT, SALMO-GAIRDNERI
Autore:
SUMI T; HAMA Y; MARUYAMA D; ASAKAWA M; NAKAGAWA H;
Indirizzi:
SAGA UNIV,FAC AGR,DEPT APPL BIOL SCI,HONJYO MACHI SAGA 840 JAPAN SAGA UNIV,FAC AGR,DEPT APPL BIOL SCI SAGA 840 JAPAN KUMAMOTO UNIV,FAC EDUC,FOOD SCI LAB KUMAMOTO 860 JAPAN
Titolo Testata:
Bioscience, biotechnology, and biochemistry
fascicolo: 4, volume: 61, anno: 1997,
pagine: 675 - 683
SICI:
0916-8451(1997)61:4<675:PACOGF>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEAMINATED NEURAMINIC ACID; RICH; KDN; POLYSIALOGLYCOPROTEIN; FLUID; EGGS;
Keywords:
SIALOGLYCOPROTEIN; MUCUS GLYCOPROTEIN; MUCUS; RAINBOW TROUT; MUCIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
T. Sumi et al., "PURIFICATION AND CHARACTERIZATION OF GLYCOPROTEIN FROM THE SKIN MUCUSOF THE RAINBOW-TROUT, SALMO-GAIRDNERI", Bioscience, biotechnology, and biochemistry, 61(4), 1997, pp. 675-683

Abstract

Mucus glycoprotein (RGP) was purified and characterized from the skinmucus of rainbow trout, Salmo gairdneri. RGP was found to contain 30.1% NeuAc, 26.0% GalNAc, 5.0% Gal, and 26.0% amino acids. The protein moiety of RGP is very rich in Thr (32.4 mol%), Neither NeuGc nor KDN (2-keto-3-deoxy-D-glycero-D-galacto-nononic acid) was found in RGP. Alkaline borohydride treatment of RGP yielded a major disaccharide alditol, NeuAc alpha 2c-->6GalNAc-ol and more than 4 minor oligosaccharide alditols including NeuAc-->(GalNAc alpha 1-->)GalNAc-ol. It was evident that an average RGP molecule has approximately 500 NeuAc-containing oligosaccharide chains, which are attached to the Thr and Ser residues of the protein moiety and spaced at an average of 3 amino acids apart.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 22:08:06