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Titolo:
DIFFERENTIAL EXPRESSION OF URINARY INTER-ALPHA-TRYPSIN INHIBITOR TRIMERS AND DIMERS IN NORMAL COMPARED TO ACTIVE CALCIUM-OXALATE STONE FORMING MEN
Autore:
MARENGO SR; RESNICK MI; YANG LZ; CHUNG JY;
Indirizzi:
SCH MED,UROL RES LABS,2109 ADELBERT RD CLEVELAND OH 44106 CASE WESTERN RESERVE UNIV,SCH MED,DEPT UROL,JAMES & EILLEEN DICKE RESLAB CLEVELAND OH 44106
Titolo Testata:
The Journal of urology
fascicolo: 5, volume: 159, anno: 1998,
pagine: 1444 - 1450
SICI:
0022-5347(1998)159:5<1444:DEOUII>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACID-RICH PROTEIN; TAMM-HORSFALL GLYCOPROTEIN; IN-VITRO; BIKUNIN; CRYSTALLIZATION; NEPHROLITHIASIS; NEPHROCALCIN; FORMERS; ITI;
Keywords:
URINARY CALCULI; TRYPSIN INHIBITORS; CALCIUM OXALATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
S.R. Marengo et al., "DIFFERENTIAL EXPRESSION OF URINARY INTER-ALPHA-TRYPSIN INHIBITOR TRIMERS AND DIMERS IN NORMAL COMPARED TO ACTIVE CALCIUM-OXALATE STONE FORMING MEN", The Journal of urology, 159(5), 1998, pp. 1444-1450

Abstract

Purpose: We determine if the immunoreactive profile of urinary inter-alpha-trypsin inhibitor can be used to distinguish between normal individuals and individuals with calcium oxalate stone disease. Materials and Methods: Urinary proteins were dialyzed against water (15 kDa. molecular weight cutoff), lyophilized and resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (6% acrylamide, reducing conditions) followed by Western blot. Inter-alpha-trypsin immunoreactive proteins were detected by enhanced chemiluminescence. Stone formation wasconfirmed to be active radiologically or passed as stone or gravel within 12 months of the sample, Stone composition was confirmed crystallographically. Normal individuals had no personal or familial history of urolithiasis and matched stone forming patients regarding race (white) and age (23 to 71 years old). Urine from a total of 101 individualswas analyzed. Results: The intact inter-alpha-trypsin trimer (similarto 220 to 240 kDa,) and heavy chain (HC) 2-bikunin/HCl-bikunin dimers(similar to 115 to 130 kDa,) were detected more often in stone forming men (23 of 26 [89%] and 26 of 26 [100%], respectively) than in normal individuals (6 of 26 [23%] and 5 of 26 [19%], respectively, p < 0.0001). In those normal individuals who expressed inter-alpha-trypsin trimer and HC-bikunins the relative intensities were 5.3 +/- 1.4% and 16.3 +/- 17.1% of the stone forming controls, respectively. The identity of high molecular weight-inter-alpha-trypsin immunoreactive bands was confirmed using antibodies against the individual subunits (HC1, HC2, HC3, bikunin). In contrast to men high molecular weight-inter-alpha-trypsin's were readily detected in normal and stone forming women with equal frequency (inter-alpha-trypsin-trimer p = 0.1337, HC-bikunins p =0.2836): inter-alpha-trypsin-trimer 17 of 18 [94%] and 9 of 13 [77%];HC-bikunins 17 of 18 [94%] and 10 of 13 [85%]). Inter-alpha-trypsin-trimer and HC-bikunins, respectively, were detected in 2 and 5 of 10 patients with chronic renal disease. Expression was not related to hematuria or proteinuria. Conclusions: Immunoreactive profiles of urinary proteins may be able to be developed into a useful diagnostic tool to identify active stone formation, although a separate panel may be required for men and women. It is possible that these differences may provide clues as to why the incidence of stone disease is higher in men than women.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 03:22:43