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Titolo:
DIFFERENTIAL EXPRESSION OF SOMATOSTATIN RECEPTORS BY QUANTITATIVE PCRIN THE RAT-BRAIN
Autore:
VIOLLET C; FAIVREBAUMAN A; ZHANG J; LLORENSCORTES C; LOUDES C; KORDON C; EPELBAUM J;
Indirizzi:
CTR PAUL BROCA,INSERM,U159,2 RUE ALESIA F-75014 PARIS FRANCE CTR PAUL BROCA,INSERM,U159 F-75014 PARIS FRANCE COLL FRANCE,INSERM,U36 F-75005 PARIS FRANCE
Titolo Testata:
Comptes rendus de l'Academie des sciences. Serie 3, Sciences de la vie
fascicolo: 8, volume: 318, anno: 1995,
pagine: 851 - 857
SICI:
0764-4469(1995)318:8<851:DEOSRB>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; MOLECULAR-CLONING; RIBONUCLEIC-ACID; MESSENGER-RNA; PITUITARY; SUBTYPES;
Keywords:
SOMATOSTATIN RECEPTOR SUBTYPES; QUANTITATIVE RT-PCR; CEREBELLUM; HYPOTHALAMUS; SPINAL CORD;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
C. Viollet et al., "DIFFERENTIAL EXPRESSION OF SOMATOSTATIN RECEPTORS BY QUANTITATIVE PCRIN THE RAT-BRAIN", Comptes rendus de l'Academie des sciences. Serie 3, Sciences de la vie, 318(8), 1995, pp. 851-857

Abstract

Five subtypes of somatostatin receptors (sst) have recently been cloned and reported to be expressed in rat brain. However, conventional mRNA measurement techniques do not allow to accurately compare the levels of expression of the 5 sst. Thus, we established a quantitative reverse transcriptase polymerase chain reaction method for the 5 sst. A cRNA internal standard was constructed by inserting in msst1 plasmid sequences corresponding to specific sense printers for amplification of each sst. Using a common reverse primer, a unique primer pair by receptor amplifies both wild type and standard RNAs with the same efficiency. The technique was validated by evaluating sst mRNAs in 3 brain structures in which different somatostatin receptor binding levels were previously reported. While the absolute level of expression is similar between regions, sst3 is the major subtype in cerebellum, sst1 predominates in spinal cord and sst4 and sst2 are equally expressed in the hypothalamus.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 19:34:35