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Titolo:
DETECTION OF MEGAKARYOCYTE COLONIES IN PLASMA CLOT CULTURES BY IMMUNOENZYMATIC STAINING
Autore:
PERUTELLI P; PASINO M; MAROTTA F; MORI PG;
Indirizzi:
G GASLINI INST CHILDREN,DEPT HEMATOL & ONCOL,HEMATOL LAB,LARGO G GASLINI 5 I-16147 GENOA ITALY
Titolo Testata:
Haematologica
fascicolo: 2, volume: 83, anno: 1998,
pagine: 189 - 190
SICI:
0390-6078(1998)83:2<189:DOMCIP>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
THROMBOCYTHEMIA; IDENTIFICATION; COMPLEX;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
10
Recensione:
Indirizzi per estratti:
Citazione:
P. Perutelli et al., "DETECTION OF MEGAKARYOCYTE COLONIES IN PLASMA CLOT CULTURES BY IMMUNOENZYMATIC STAINING", Haematologica, 83(2), 1998, pp. 189-190

Abstract

In vitro induced megakaryocytic differentiation/maturation of megakayocyte (meg) progenitors rep resents an important tool for investigating cytokine-induced in vitro thrombocytopoiesis.(1-3) We have developedan assay which allows the in situ study of human meg progenitor-derived colonies, cultured on a plasma clot in the presence of cytokines. Plates were immunostained by using an anti-alpha IIb beta 3 monoclonal antibody and an alkaline phosphatase-labeled secondary antibody. alphaIIb beta 3-bearing cells were stained an intense red and were clearlydifferentiated from the negative cells. Processed plates were stable for some weeks at 4 degrees C. The described procedure is easy to perform and allowed us to enumerate the meg colonies and assess colony morphology and cell ploidy.

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Documento generato il 11/07/20 alle ore 19:28:03