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Titolo:
IMMUNOHISTOCHEMICAL STAINING FOR DESMOGLEINS-1 AND DESMOGLEIN-2 IN KERATINOCYTIC NEOPLASMS WITH SQUAMOUS PHENOTYPE - ACTINIC KERATOSIS, KERATOACANTHOMA AND SQUAMOUS-CELL CARCINOMA OF THE SKIN
Autore:
KRUNIC AL; GARROD DR; MADANI S; BUCHANAN MD; CLARK RE;
Indirizzi:
UNIV MANCHESTER,SCH BIOL SCI,EPITHELIAL MORPHOGENESIS RES GRP,3-239 STOPFORD BLDG,OXFORD RD MANCHESTER M13 9PT LANCS ENGLAND UNIV MANCHESTER,SCH BIOL SCI,EPITHELIAL MORPHOGENESIS RES GRP MANCHESTER M13 9PT LANCS ENGLAND DUKE UNIV,MED CTR,DERMATOL SURG UNIT DURHAM NC 27710
Titolo Testata:
British Journal of Cancer
fascicolo: 8, volume: 77, anno: 1998,
pagine: 1275 - 1279
SICI:
0007-0920(1998)77:8<1275:ISFDAD>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
BCL-2 EXPRESSION; HETEROZYGOSITY; TUMORS; DIFFERENTIATION; ASSOCIATION; DESMOSOMES; DIAGNOSIS; CADHERIN; DISEASE; ANTIGEN;
Keywords:
DESMOGLEIN; KERATOACANTHOMA; SQUAMOUS CELL CARCINOMA; ACTINIC KERATOSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
A.L. Krunic et al., "IMMUNOHISTOCHEMICAL STAINING FOR DESMOGLEINS-1 AND DESMOGLEIN-2 IN KERATINOCYTIC NEOPLASMS WITH SQUAMOUS PHENOTYPE - ACTINIC KERATOSIS, KERATOACANTHOMA AND SQUAMOUS-CELL CARCINOMA OF THE SKIN", British Journal of Cancer, 77(8), 1998, pp. 1275-1279

Abstract

Desmosomes are intercellular junctions that have been shown to be down-regulated in certain types of carcinoma and that may play a role in suppression of invasion and metastasis. This paper describes an immunohistochemical study of three types of epidermal neoplasms with monoclonal antibody to desmoglein in order to determine how desmosomal staining correlates with the clinical, biological and histopathological features of these neoplasms. Actinic keratosis (AK) is the most common keratinocytic premalignant neoplasm that was reported to have a 10-20% rate of malignant transformation into squamous cell carcinoma (SCC). Keratoacanthoma (KA) is a benign neoplasm that involutes spontaneously after a few months of rapid growth. SCC is a malignant tumour capable ofmetastasis. Electron microscope studies of KA and SCC showed significantly reduced staining for desmosomes in SCC but not in KA. We have examined staining for desmoglein using the monoclonal antibody 33-3D, a mouse IgM monoclonal antibody, that recognizes the cytoplasmic domainsof desmoglein (Dsg)1 and Dsg2 on frozen sections. Immunohistochemicalstaining of normal skin with this antibody revealed strong pericellular localization of the antigen, outlining the cell membranes of the keratinocytes. A series of 30 AKs, 12 KAs and 24 SCCs was stained immunohistochemically with 33-3D monoclonal antibody. All examined KAs showed extensive pericellular staining for Dsg. By contrast, juxtanuclear staining for Dsg was noted in 12 SCCs, and completely negative stainingin seven SCCs. The five remaining SCCs showed focal pericellular staining for the Dsg marker. The most common finding in AK was focal pericellular staining for Dsg, with complete absence of staining in dysplastic areas (25 cases). in five cases negative pericellular staining in dysplastic areas was associated with juxtanuclear accumulation of the Dsg marker. A strong negative correlation between Dsg staining and degree of dysplasia was obtained. The Dsg pattern in KA is similar to normal epidermis and shows a clear difference between KA and SCC. AK has a limited loss of Dsg expression in a SCC-like pattern that is congruent with its premalignant nature. As the stain works on frozen tissue, it may be helpful for rapid differentiation in selected cases in cutaneous oncology and Mohs micrographic surgery. This antibody may also have great potential for the detection of the effects of chemopreventiveagents in skin cancer.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 10:42:20