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Titolo:
STRUCTURE OF THE O-GLYCOPEPTIDES ISOLATED FROM BOVINE-MILK COMPONENT PP3
Autore:
CODDEVILLE B; GIRARDET JM; PLANCKE Y; CAMPAGNA S; LINDEN G; SPIK G;
Indirizzi:
UNIV SCI & TECH LILLE FLANDRES ARTOIS,CHIM BIOL LAB,CNRS,UMR 111 F-59655 VILLENEUVE DASCQ FRANCE UNIV SCI & TECH LILLE FLANDRES ARTOIS,CHIM BIOL LAB,CNRS,UMR 111 F-59655 VILLENEUVE DASCQ FRANCE UNIV HENRI POINCARE,FAC SCI,LAB BIOSCI ALIMENT,INRA F-54506 VANDOEUVRE NANCY FRANCE
Titolo Testata:
Glycoconjugate journal
fascicolo: 4, volume: 15, anno: 1998,
pagine: 371 - 378
SICI:
0282-0080(1998)15:4<371:SOTOIF>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAS-LIQUID-CHROMATOGRAPHY; FAT GLOBULE-MEMBRANE; MAGNETIC-RESONANCE SPECTROSCOPY; LINKED CARBOHYDRATE CHAINS; CELL-ADHESION MOLECULE-1; SIALYL-LEWIS-X; L-SELECTIN; KAPPA-CASEIN; ENDOTHELIAL LIGAND; METHYL GLYCOSIDES;
Keywords:
BOVINE MILK; LACTOPHORIN; PROTEOSE PEPTONE; COMPONENT PP3; O-GLYCAN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
B. Coddeville et al., "STRUCTURE OF THE O-GLYCOPEPTIDES ISOLATED FROM BOVINE-MILK COMPONENT PP3", Glycoconjugate journal, 15(4), 1998, pp. 371-378

Abstract

The heat-stable acid-soluble phosphoglycoprotein component PP3 was isolated from the bovine milk proteose peptone fraction by concanavalin A affinity chromatography. Glycopeptides from the ConA-bound fraction corresponding to the component PP3 were obtained by Pronase digestion and were separated by gel filtration into high and low-molecular-mass glycopeptides. In a previous work, we have investigated the structure of the N-glycans from the high-molecular-mass glycopeptides [Girardet et al. (1995) Eur J Biochem 234: 939-46]. Here, we describe the structure of the O-glycans from the low-molecular-mass glycopeptides. By combining methylation analysis, mass spectrometry, 400 MHz H-1-NMR spectroscopy and peptide sequence analysis, we show that the low-molecular-mass fraction contains several neutral glycopeptides. A mixture of the following three glycan structures linked to the Thr(86) has been identified: GalNac alpha 1-O-Thr, Gal(beta 1-3)GalNAc alpha 1-O-Thr and Gal(beta 1-4)GlcNAc(beta 1-6)[Gal(beta 1-3)]GalNAc alpha 1-O-Thr.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 13:22:52