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Titolo:
STRESS RESPONSIVENESS OF THE PITUITARY-INTERRENAL AXIS DURING EARLY-LIFE STAGES OF COMMON CARP (CYPRINUS-CARPIO)
Autore:
STOUTHART AJHX; LUCASSEN ECHET; VANSTRIEN FJC; BALM PHM; LOCK RAC; BONGA SEW;
Indirizzi:
CATHOLIC UNIV NIJMEGEN,FAC SCI,DEPT ANIM PHYSIOL,TOERNOOIVELD 1 NL-6525 ED NIJMEGEN NETHERLANDS
Titolo Testata:
Journal of Endocrinology
fascicolo: 1, volume: 157, anno: 1998,
pagine: 127 - 137
SICI:
0022-0795(1998)157:1<127:SROTPA>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
TILAPIA OREOCHROMIS-MOSSAMBICUS; MELANOCYTE-STIMULATING HORMONE; WHOLE-BODY CONCENTRATIONS; RAINBOW-TROUT; ONCORHYNCHUS-KETA; THYROID-HORMONES; CHUM SALMON; CORTISOL; ONTOGENY; LARVAE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
A.J.H.X. Stouthart et al., "STRESS RESPONSIVENESS OF THE PITUITARY-INTERRENAL AXIS DURING EARLY-LIFE STAGES OF COMMON CARP (CYPRINUS-CARPIO)", Journal of Endocrinology, 157(1), 1998, pp. 127-137

Abstract

Whole-body levels of ACTH, alpha-MSH and cortisol in eggs and larvae of the common carp (Cyprinus carpio) were determined periodically up until 168 h after fertilisation. ACTH, alpha-MSH and cortisol immunoreactivity was detected in unfertilised eggs, and endogenous production of ACTH and alpha-MSH was observed 24 h after fertilisation and that ofcortisol 36 h after fertilisation. ACTH immunoreactivity reached peaklevels before hatching (56-72 h after fertilisation) and remained relatively stable thereafter, while alpha-MSH immunoreactivity started toincrease after hatching. At 36 h after fertilisation, whole-body cortisol levels increased rapidly reaching peak levels at the end of hatching (72 h after fertilisation), remaining stable until the end of the experiment. From 50 h after fertilisation onwards, embryos and larvae increased their whole-body cortisol levels when subjected to handling (mechanical pressure during egg stage or netting during the larval stage). It is concluded that the pituitary-interrenal axis in carp is fully functional at the time of hatching. No indications of a stress non-responsive period after hatching were observed. To characterise ACTH and alpha-MSH immunoreactivities in carp larvae, whole-body homogenateswere analysed by HPLC, with pituitary homogenates of adult carp serving as a reference. ACTH and alpha-MSH immunoreactivity in carp larvae homogenates consisted of three and two products respectively. HPLC of adult carp pituitaries revealed the presence of two ACTH immunoreactive products, which may represent a phosphorylated and a non-phosphorylated ACTH variant, while the three alpha-MSH peaks most likely represent des-acetylated, mono-acetylated and di-acetylated alpha-MSH, the latter being the predominant form. In carp larvae, however, one of the ACTH immunoreactive products co-eluted with the non-phosphorylated ACTH,while the two alpha-MSH products identified co-eluted with des-acetylated and mono-acetylated alpha-MSH, indicating that POMC processing atthis stage of development is different from prohormone processing in adult fish.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:56:06