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Titolo:
EXPRESSION OF CD38 INCREASES INTRACELLULAR CALCIUM-CONCENTRATION AND REDUCES DOUBLING TIME IN HELA AND 3T3 CELLS
Autore:
ZOCCHI E; DAGA A; USAI C; FRANCO L; GUIDA L; BRUZZONE S; COSTA A; MARCHETTI C; DEFLORA A;
Indirizzi:
UNIV GENOA,INST BIOCHEM,VIALE BENEDETTO XV 1 I-16132 GENOA ITALY ADV BIOTECHNOL CTR I-16132 GENOA ITALY NATL RES COUNCIL,INST CYBERNET & BIOPHYS I-16149 GENOA ITALY IST NAZL STUDIO & CURA TUMORI I-20133 MILAN ITALY
Titolo Testata:
The Journal of biological chemistry
fascicolo: 14, volume: 273, anno: 1998,
pagine: 8017 - 8024
SICI:
0021-9258(1998)273:14<8017:EOCIIC>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLIC ADP-RIBOSE; TRANSMEMBRANE GLYCOPROTEIN CD38; TYROSINE PHOSPHORYLATION; HUMAN ERYTHROCYTES; ANTIGEN CD38; B-CELLS; SURFACE; NUCLEOTIDES; ACTIVATION; HYDROLYSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
E. Zocchi et al., "EXPRESSION OF CD38 INCREASES INTRACELLULAR CALCIUM-CONCENTRATION AND REDUCES DOUBLING TIME IN HELA AND 3T3 CELLS", The Journal of biological chemistry, 273(14), 1998, pp. 8017-8024

Abstract

CD38 is a bifunctional ectoenzyme, predominantly expressed on hematopoietic cells during differentiation, that catalyzes the synthesis (cyclase) and the degradation (hydrolase) of cyclic ADP-ribose (cADPR), a powerful calcium mobilizer from intracellular stores, Due to the well established role of calcium levels in the regulation of apoptosis, proliferation, and differentiation, the CD38/cADPR system seems to be a likely candidate involved in the control of these fundamental processes. The ectocellular localization of the cyclase activity, however, contrasts with the intracellular site of action of cADPR. Here we demonstrate that ectocellular expression of human CD38 in CD38(-) HeLa and 3T3cells results in intracellular CD38 substrate (NAI(+) + NADH) consumption and product (cADPR) accumulation. Furthermore, a causal relationship is established between presence of intracellular cADPR, partial depletion of thapsigargin-sensitive calcium stores, increase in basal free cytoplasmic calcium concentration, and decrease of cell doubling time. The significant shortening of the S phase in CD38(+) HeLa cells, as compared with controls, demonstrates an effect of intracellular cADPR on the mammalian cell cycle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/10/20 alle ore 09:53:30