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Titolo:
PARATHYROID-HORMONE REGULATES THE EXPRESSION OF THE NUCLEAR MITOTIC APPARATUS PROTEIN IN THE OSTEOBLAST-LIKE CELLS, ROS-17 2.8/
Autore:
TORRUNGRUANG K; FEISTER H; SWARTZ D; HANCOCK EB; HOCK J; BIDWELL JP;
Indirizzi:
INDIANA UNIV,SCH DENT,DEPT PERIODONT,DS235,1121 W MICHIGAN ST INDIANAPOLIS IN 46202 INDIANA UNIV,SCH DENT,DEPT PERIODONT INDIANAPOLIS IN 46202 INDIANA UNIV,SCH DENT,DEPT ANAT INDIANAPOLIS IN 46202 ELI LILLY & CO,DIV ENDOCRINE INDIANAPOLIS IN 46285
Titolo Testata:
Bone
fascicolo: 4, volume: 22, anno: 1998,
pagine: 317 - 324
SICI:
8756-3282(1998)22:4<317:PRTEOT>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOSKELETAL ACTIN; MATRIX PROTEINS; CYCLIC-AMP; NUMA; DNA; BONE; TRANSCRIPTION; CALCIUM; TOPOISOMERASES; FIBROBLASTS;
Keywords:
CONFOCAL MICROSCOPY; NUCLEAR MATRIX; NUCLEAR MITOTIC APPARATUS PROTEIN; OSTEOBLAST-LIKE CELLS; PARATHYROID HORMONE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
K. Torrungruang et al., "PARATHYROID-HORMONE REGULATES THE EXPRESSION OF THE NUCLEAR MITOTIC APPARATUS PROTEIN IN THE OSTEOBLAST-LIKE CELLS, ROS-17 2.8/", Bone, 22(4), 1998, pp. 317-324

Abstract

The parathyroid hormone (PTH) signaling pathways that effect changes in osteoblast gene expression also alter the organization of the cytoskeletal proteins, PTH regulates the expression of nucleoskeletal proteins, such as nuclear mitotic apparatus protein (NuMA) and topoisomerase II-alpha. NuMA is a structural component of the interphase nucleus and organizes the microtubules of the mitotic spindle during mitogenesis, We propose that PTH-induced alterations in osteoblast cytoarchitecture are accompanied by changes in osteoblast nuclear structure that contribute to changes in gene expression, We used immunofluorescence andconfocal microscopy to determine the effect of PTH on the expression and nuclear distribution of NuMA in the rat osteosarcoma cell line, ROS 17/2.8, Cells were treated with PTH or vehicle, then fixed and stained with NuMA antibody, Optical sections of interphase naive cells revealed a diffuse distribution of NuMA, interspersed with speckles, in the central nuclear planes but not in nucleoli, During the metaphase andanaphase, NuMA localized at the mitotic spindle apparatus, The percentage of NuMA-immunopositive ROS 17/2.8 cells decreased with increasingconfluence, but serum starvation did not attenuate NuMA expression, Cell density-dependent changes in cytoskeletal organization were observed in these cells, PTH treatment induced changes in cytoskeletal organization and increased the percentage of NuMA-immunopositive ROS 17/2.8cells, These data suggest that PTH effects changes in osteoblast nuclear architecture by regulating NuMA, and that these alterations may becoupled to cytoskeletal organization, (C) 1998 by Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 11:31:52