Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
FTIR MICROSPECTROSCOPIC STUDY OF CELL-TYPES AND POTENTIAL CONFOUNDINGVARIABLES IN SCREENING FOR CERVICAL MALIGNANCIES
Autore:
WOOD BR; QUINN MA; TAIT B; ASHDOWN M; HISLOP T; ROMEO M; MCNAUGHTON D;
Indirizzi:
MONASH UNIV,DEPT CHEM,WELLINGTON RD CLAYTON VIC 3168 AUSTRALIA MONASH UNIV,DEPT CHEM CLAYTON VIC 3168 AUSTRALIA UNIV MELBOURNE,DEPT OBSTET & GYNAECOL PARKVILLE VIC 3052 AUSTRALIA ROYAL MELBOURNE HOSP,DEPT TISSUE TYPING PARKVILLE VIC 3052 AUSTRALIA
Titolo Testata:
Biospectroscopy
fascicolo: 2, volume: 4, anno: 1998,
pagine: 75 - 91
SICI:
1075-4261(1998)4:2<75:FMSOCA>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSFORM INFRARED-SPECTROSCOPY; RAMAN-SPECTROSCOPY; BIOPSY SPECIMENS; IR SPECTROSCOPY; HUMAN TISSUES; BACTERIA; IDENTIFICATION; SPECTRA; MRS;
Keywords:
FTIR MICROSPECTROSCOPY; CERVICAL CANCER; LEUKOCYTES; LYMPHOCYTES; ERYTHROCYTES; SEMEN; MUCINS; FIBROBLASTS; THROMBOCYTES; BACTERIA; NYLON; CANDIDA ALBICANS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
B.R. Wood et al., "FTIR MICROSPECTROSCOPIC STUDY OF CELL-TYPES AND POTENTIAL CONFOUNDINGVARIABLES IN SCREENING FOR CERVICAL MALIGNANCIES", Biospectroscopy, 4(2), 1998, pp. 75-91

Abstract

FTIR microscopy was applied to the analysis of cell types and other variables present in Pap smears to ascertain the limitations of infrared spectroscopy in the diagnosis of cervical cancer and dysplasia. It was found that leukocytes, and in particular lymphocytes, have spectralfeatures in the phophodiester region (1300-900 cm(-1)) suggestive of what has previously been described as changes indicative of malignancy. Endocervical cells and fibroblasts have similar spectral features toHeLa cells and consequently could also confound diagnosis. The use ofethanol as a fixative and dehydrating agent results in retention of glycogen in cervical cell types and thus minimizes spectral changes in the glycogen region due to sampling technique. Spectra of seminal fluids exhibit strong bands in the phosphodiester/carbohydrate region; however, sperm contamination should be easily detectable by the presence of a distinctive doublet at 981/968 cm(-1). Erythrocyte spectra exhibit a reduction in glycogen band intensity, but can be discerned by a relatively low-intensity upsilon(s) PO2- band. Endocervical mucin spectra exhibit a reduction in glycogen bands and a very pronounced upsilon(s) PO2- band, which is similar in intensity to the corresponding band in HeLa cells. Thrombocytes have strong bands in the phosphodiester region, but thrombocytes can be discerned from other cell types by the presence of two small broad bands at 980 and 935 cm(-1). Candida albicans is characterized by strong bands in the polysaccharide region whichcould potentially obscure diagnostic bands if C. albicans is present in large numbers. Spectra of bacteria common to the female genital tract, in general, also have strong absorptions in the polysaccharide region; however, bacterial contamination is usually minimal and would notbe expected to obscure cervical cell spectra. Nylon threads and bristles from cervical sampling implements produce characteristic IR profiles which allow for easy identification. Given the number of potential confounding variables associated with cervical cytology, a multivariate statistical or neural network analysis would appear to be necessary before the implementation of FTIR technology in clinical laboratories. (C) 1998 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/05/20 alle ore 14:49:53