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Titolo:
SPECIFIC BINDING-SITES FOR [H-3]DEXAMETHASONE IN THE HYPOTHALAMUS OF JUVENILE RAINBOW-TROUT, ONCORHYNCHUS-MYKISS
Autore:
ALLISON CM; OMELJANIUK RJ;
Indirizzi:
LAKEHEAD UNIV,DEPT BIOL,955 OLIVER RD THUNDER BAY ON P7B 5E1 CANADA
Titolo Testata:
General and comparative endocrinology
fascicolo: 1, volume: 110, anno: 1998,
pagine: 2 - 10
SICI:
0016-6480(1998)110:1<2:SBF[IT>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
SALMO-TRUTTA-L; CORTISOL RECEPTOR ACTIVITY; PITUITARY-INTERRENAL AXIS; GLUCOCORTICOID RECEPTORS; SALVELINUS-FONTINALIS; CONSCIOUS RATS; BROOK TROUT; BROWN TROUT; STRESS; BRAIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
C.M. Allison e R.J. Omeljaniuk, "SPECIFIC BINDING-SITES FOR [H-3]DEXAMETHASONE IN THE HYPOTHALAMUS OF JUVENILE RAINBOW-TROUT, ONCORHYNCHUS-MYKISS", General and comparative endocrinology, 110(1), 1998, pp. 2-10

Abstract

Indirect evidence suggests that glucocoriticoid hormone's may act through cellular receptors to play a neuromodulatory role in the teleost CNS. We now report our findings on the use of [H-3]dexamethasone (DEX)to identify hypothalamic glucocorticoid receptors (GRs) in juvenile rainbow trout, Oncorhynchus mykiss. Hypothalamic cytosol was incubated with [H-3]DEX under various experimental paradigms with incubations terminated by addition of dextran-coated charcoal; following immediate centrifugation, a sample of bound [H-3]DEX (supernatant) was collected and assessed for H-3 content. [H-3]DEX binding was tissue dependent between 0.5 and 2.0 hypothalamus equivalents per tube (1.0 to 4.7 ng protein, respectively). Specific binding (B-SP) increased with time for 1.5 h and remained relatively constant for an additional 2.5 h; the calculated association rate constant was 2.23 x 10(8) M-1 x min(-1). Equilibrium B-SP was dissociated by addition of a 5000 M excess cortisol with an accompaning t(1/2) of 1.25 h and dissociation rate constant of 0.553 min(-1). B-SP was saturable with a calculated equilibrium K-d and B-MAX of 1.22 nM and 296 fmol/mg protein, respectively. B-SP was displaced under equilibrium conditions by the corticosteroids, but to a lesser extent by the mineralocorticoid, estrogen, and progestin. The rank order of potency for [H-3]DEX displacement was DEX > cortisol >> corticosterone > m triamcinolone = 11-deoxycortisol >> aldosterone > progesterone >>> 17 beta-estradiol. These properties of specifically bound [H-3]DEX indicate the presence of a GR, similar to the mammalian cytosolic GR, in the hypothalamus of juvenile rainbow trout. (C) 1998 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 19:31:37