Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
PARALLEL ANALYSIS OF GENETIC SELECTIONS USING WHOLE GENOME OLIGONUCLEOTIDE ARRAYS
Autore:
CHO RJ; FROMONTRACINE M; WODICKA L; FEIERBACH B; STEARNS T; LEGRAIN P; LOCKHART DJ; DAVIS RW;
Indirizzi:
STANFORD UNIV,SCH MED,DEPT GENET STANFORD CA 94305 STANFORD UNIV,SCH MED,DEPT BIOL STANFORD CA 94305 INST PASTEUR,LAB METAB ARN,CNRS,URA 1300 F-75724 PARIS 15 FRANCE AFFYMETRIX,DEPT GENOM RES SANTA CLARA CA 95051
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 7, volume: 95, anno: 1998,
pagine: 3752 - 3757
SICI:
0027-8424(1998)95:7<3752:PAOGSU>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN INTERACTIONS; ESCHERICHIA-COLI; 2-HYBRID SYSTEM; YEAST;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
R.J. Cho et al., "PARALLEL ANALYSIS OF GENETIC SELECTIONS USING WHOLE GENOME OLIGONUCLEOTIDE ARRAYS", Proceedings of the National Academy of Sciences of the United Statesof America, 95(7), 1998, pp. 3752-3757

Abstract

Thousands of genes have recently been sequenced in organisms ranging from Escherichia coli to human. For the majority of these genes, however, available sequence does not define a biological role, Efficient functional characterization of these genes requires strategies for scaling genetic analyses to the whole genome level. Plasmid-based library selections are an established approach to the functional analysis of uncharacterized genes and can help elucidate biological function by identifying, for example, physical interactors for a gene and genetic enhancers and suppressors of mutant phenotypes. The application of these selections to every gene in a eukaryotic genome, however, is generally limited by the need to manipulate and sequence hundreds of DNA plasmids, We present an alternative approach in which identification of nucleic acids is accomplished by direct hybridization to high-density oligonucleotide arrays. Based on the complete sequence of Saccharomyces cerevisiae, high-density arrays containing oligonucleotides complementaryto every gene in the yeast genome have been designed and synthesized,Two-hybrid protein-protein intel action screens were carried out for S. cerevisiae genes implicated in mRNA splicing and microtubule assembly. Hybridization of labeled DNA derived from positive clones is sufficient to characterize the results of a screen in a single experiment, allowing rapid determination of both established and previously unknown biological interactions, These results demonstrate the use of oligonucleotide arrays for the analysis of two-hybrid screens, This approachshould be generally applicable to the analysis of a range of genetic selections.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 13:07:02