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Titolo:
PEROXYNITRITE INDUCES APOPTOSIS OF HL-60 CELLS BY ACTIVATION OF A CASPASE-3 FAMILY PROTEASE
Autore:
LIN KT; XUE JY; LIN MC; SPOKAS EG; SUN FF; WONG PYK;
Indirizzi:
UNIV MED & DENT NEW JERSEY,SCH OSTEOPATH MED,DEPT CELL BIOL STRATFORDNJ 08084 UNIV MED & DENT NEW JERSEY,SCH OSTEOPATH MED,DEPT CELL BIOL STRATFORDNJ 08084 UNIV MED & DENT NEW JERSEY,ROBERT WOOD JOHNSON MED SCH,DEPT MOL GENET& MICROBIOL PISCATAWAY NJ 08854
Titolo Testata:
American journal of physiology. Cell physiology
fascicolo: 4, volume: 43, anno: 1998,
pagine: 855 - 860
SICI:
0363-6143(1998)43:4<855:PIAOHC>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE SYNTHASE; POLY(ADP-RIBOSE) POLYMERASE; ADP-RIBOSYLATION; MAMMALIAN HOMOLOG; ENERGY DEPLETION; HUMAN PLATELETS; INDUCTION; CLEAVAGE; DEATH; DNA;
Keywords:
PROGRAMMED CELL DEATH; LEUKEMIA CELLS; REACTIVE NITROGEN SPECIES; CASPASE CYSTEINE PROTEASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
K.T. Lin et al., "PEROXYNITRITE INDUCES APOPTOSIS OF HL-60 CELLS BY ACTIVATION OF A CASPASE-3 FAMILY PROTEASE", American journal of physiology. Cell physiology, 43(4), 1998, pp. 855-860

Abstract

Apoptosis is an active process critical for the homeostasis of organisms. Enzymes of the caspase family are responsible for executing this process. We have previously shown that peroxynitrite (ONOO-), a biological product generated from the interaction of nitric oxide and superoxide, induces apoptosis of HL-60 cells. The aim of this study was to elucidate the mechanisms involved in the execution process of peroxynitrite-induced apoptosis. Proteolytic cleavage of poly(ADP-ribose) polymerase, an indication of caspase-3 family protease activation and an early biochemical event accompanying apoptosis, was observed in a time-dependent manner during peroxynitrite-induced apoptosis of HL-60 cells. Activation of caspase-3 during peroxynitrite-induced apoptosis was substantiated by monitoring proteolysis of the caspase-3 proenzyme and by measuring caspase-3 activity with a fluorogenic substrate. Furthermore, pretreatment of HL-60 cells with N-acetyl-Asp-Glu-Val-Asp-aldehyde, a specific inhibitor of caspase-3, but not N-acetyl-Tyr-Val-Ala-Asp-aldehyde, a specific inhibitor of caspase-1, decreased peroxynitrite-induced apoptosis. These results suggest that the activation of a caspase-3 family protease is essential for initiating the execution processof peroxynitrite-induced apoptosis of HL-60 cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 22:14:50