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Titolo:
GAMMA-DELTA(-BETA(+) HUMAN T-CELL SUBSET RESPONSES UPON STIMULATION WITH VARIOUS MYCOBACTERIUM-TUBERCULOSIS SOLUBLE EXTRACTS() AND CD4(+) ALPHA)
Autore:
BATONI G; ESIN S; HARRIS RA; KALLENIUS G; SVENSON SB; ANDERSSON R; CAMPA M; WIGZELL H;
Indirizzi:
UNIV PISA,DEPT BIOMED,VIA S ZENO 35-39 I-56127 PISA ITALY UNIV PISA,DIPARTIMENTO BIOMED SPERIMENTALE INFETT & PUBL PISA ITALY KAROLINSKA INST,SWEDISH INST INFECT DIS CONTROL STOCKHOLM SWEDEN KAROLINSKA INST,MICROBIOL & TUMOURBIOL CTR STOCKHOLM SWEDEN
Titolo Testata:
Clinical and experimental immunology
fascicolo: 1, volume: 112, anno: 1998,
pagine: 52 - 62
SICI:
0009-9104(1998)112:1<52:GHTSRU>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEAT-SHOCK PROTEIN; LISTERIA-MONOCYTOGENES; ALPHA-BETA; LYMPHOCYTES BEARING; PROTECTIVE ROLE; INFECTION; RECEPTOR; GAMMA/DELTA; ACTIVATION; IMMUNITY;
Keywords:
MYCOBACTERIUM TUBERCULOSIS; GAMMA-DELTA(+) T CELL; CD4(+) T CELL; T CELL SUBSETS; PROLIFERATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
G. Batoni et al., "GAMMA-DELTA(-BETA(+) HUMAN T-CELL SUBSET RESPONSES UPON STIMULATION WITH VARIOUS MYCOBACTERIUM-TUBERCULOSIS SOLUBLE EXTRACTS() AND CD4(+) ALPHA)", Clinical and experimental immunology, 112(1), 1998, pp. 52-62

Abstract

By using a flow cytometric technique which allows direct identification of proliferating cells within mixed cell populations, we have previously described that soluble extracts obtained from Mycobacterium tuberculosis or M. avium represent strong stimuli for human gamma delta(+)T cells. In the present study, we demonstrate that the protocol used for the preparation of M. tuberculosis soluble extracts may have an impact on their gamma delta(+) T cell stimulatory capacity. In agreementwith our previous data, soluble extracts prepared from bacteria killed at 85 degrees C and directly disrupted by prolonged sonication (TBe), elicited a strong proliferation of gamma delta(+) T cells after 6-7 days of stimulation. In contrast, when soluble extracts were obtained from bacteria autoclaved (121 degrees C, 25 min) and then washed by centrifugation, a predominant proportion of CD4(+) alpha beta(+) T cellswas achieved in the responding population. The stimulatory activity for gamma delta(+) T cells was recovered in the supernatant of the autoclaved bacteria, indicating that autoclaving of M. tuberculosis bacilli releases an antigen(s) into the supernatant which stimulates human gamma delta(+) T cells. While protease digestion of TBe only partially reduced its stimulatory capacity on gamma delta(+) T cells, the stimulatory component(s) released into the supernatant after autoclavation of bacilli was found to be sensitive to protease digestion. Interestingly, in contrast to the preponderant proportion of gamma delta(+) T cells induced in the responding population by unfractionated TBe, when the extract was fractionated by fast performance liquid chromatography (FPLC), most of the fractions exhibited a strong stimulatory capacity on CD4(+) alpha beta(+) T cells only. The gamma delta(+) T cell stimulatory activity was confined to the low molecular weight range FPLC fractions. Such results may suggest a possible regulatory role of gamma delta(+) T cells on CD4(+) alpha beta(+) T cells.

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Documento generato il 07/04/20 alle ore 22:52:37