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Titolo:
ENZYME-IMMUNOASSAY FOR CHOLESTERYL ESTER TRANSFER PROTEIN IN HUMAN SERUM
Autore:
KIYOHARA T; KIRIYAMA R; ZAMMA S; INAZU A; KOIZUMI J; MABUCHI H; CHICHIBU K;
Indirizzi:
CHUGAI PHARMACEUT CO LTD,DIABET RES LABS,DIAGNOST LAB,TOSHIMA KU,41-8TAKADA 3 CHOME TOKYO 171 JAPAN KANAZAWA UNIV,SCH MED,DEPT INTERNAL MED 2 KANAZAWA ISHIKAWA 920 JAPAN
Titolo Testata:
Clinica chimica acta
fascicolo: 2, volume: 271, anno: 1998,
pagine: 109 - 118
SICI:
0009-8981(1998)271:2<109:EFCETP>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
DENSITY-LIPOPROTEIN CHOLESTEROL; HUMAN-PLASMA; TRANSGENIC MICE; PURIFICATION; ANTIBODY;
Keywords:
CHOLESTERYL ESTER TRANSFER PROTEIN (CETP); SANDWICH IMMUNOASSAY; GENETIC DEFICIENCY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
T. Kiyohara et al., "ENZYME-IMMUNOASSAY FOR CHOLESTERYL ESTER TRANSFER PROTEIN IN HUMAN SERUM", Clinica chimica acta, 271(2), 1998, pp. 109-118

Abstract

We developed a new simple sandwich-type enzyme immmunoassay to measure cholesteryl ester transfer protein (CETP) mass in human serum. In assay validation, Intra-and Inter-assay coefficients of variation were 2.7 to 5.7% and 2.2 to 12.2%, respectively. There was no cross-reactivity with various lipoproteins (ape A-I, apo A-II, apo B, apo C-III), A good correlation between CETP mass and CETP activity (n = 46, correlation coefficient = 0.88) was observed. This assay provided a specific and reproducible method for measuring CETP mass in samples. The averagevalue of CETP in the normal sera of 41 males was 1.8+/-0.6 mu g/ml (mean+/-S.D.) and that of 37 females was 2.0+/-0.5 mu g/ml. In the studyof patients with the CETP gene mutation (Int 14A and D442G), our results on the value of plasma CETP mass reflected to genetic CETP deficiency. In conclusion, this assay for CETP mass in human serum may be a useful tool for clinical investigations involving lipid metabolism related to disease. (C) 1998 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 01:30:29