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Titolo:
CYTOLOGICAL COMPARISON OF LEAVES AND STEMS OF PRUNUS-AVIUM L. SHOOTS CULTURED ON A SOLID MEDIUM WITH AGAR OR GELRITE
Autore:
FRANCK T; CREVECOEUR M; WUEST J; GREPPIN H; GASPAR T;
Indirizzi:
UNIV LIEGE,INST BOT B-4000 LIEGE BELGIUM UNIV CTR,RES PUBL CTR L-1511 LUXEMBOURG LUXEMBOURG UNIV GENEVA,LAB PLANT BIOCHEM & PHYSIOL CH-1211 GENEVA 4 SWITZERLAND MUSEUM NATL HIST,LAB MEB CH-1211 GENEVA 4 SWITZERLAND
Titolo Testata:
Biotechnic & histochemistry
fascicolo: 1, volume: 73, anno: 1998,
pagine: 32 - 43
SICI:
1052-0295(1998)73:1<32:CCOLAS>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
VITRIFIED SHOOTS; INVITRO; VITRIFICATION; CHLOROPLAST; GROWTH; MICROPROPAGATION; DEGRADATION; PLANTLETS; DIANTHUS;
Keywords:
GELRITE; LIGHT AND ELECTRON MICROSCOPY; PRUNUS AVIUM L.; VITRIFICATION; WILD CHERRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
T. Franck et al., "CYTOLOGICAL COMPARISON OF LEAVES AND STEMS OF PRUNUS-AVIUM L. SHOOTS CULTURED ON A SOLID MEDIUM WITH AGAR OR GELRITE", Biotechnic & histochemistry, 73(1), 1998, pp. 32-43

Abstract

An axillary proliferating clone of Prunus avium L. was subcultured every four weeks on solid MS medium with agar as the gelling agent, Vitrification (hyperhydricity) of shoots was induced in one four week cycle with the same medium except that agar was replaced by gel-rite, During culture on the vitrifying medium, the water content of the shoots progressively increased with a parallel decrease in chlorophyll content, Cytological differences between the leaves and stems of the vitrified and normal shoots were detected by light and electron (both transmission and scanning) microscopy, Leaves of vitrified shoots were characterized by lower number of chloroplasts in the palisade parenchyma and by a defective cuticle, The stems of vitrified shoots had a less developed and lignified xylem tissue, lacked sclerenchymatic areas and showed hypertrophy of the cortical parenchyma, More intense vacuolar activity with evaginations of the chloroplast envelope into the vacuole wasnoted in cells of vitrified leaves.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 18:09:44