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Titolo:
ENGINEERING PASSIVE-IMMUNITY IN TRANSGENIC MICE SECRETING VIRUS-NEUTRALIZING ANTIBODIES IN MILK
Autore:
CASTILLA J; PINTADO B; SOLA I; SANCHEZMORGADO JM; ENJUANES L;
Indirizzi:
CSIC,CTR NACL BIOTECNOL,DEPT MOL & CELL BIOL,CAMPUS UNIV AUTONOMA MADRID 28049 SPAIN CSIC,CTR NACL BIOTECNOL,DEPT MOL & CELL BIOL MADRID 28049 SPAIN INST NACL INVEST AGR,DEPT ANIM REPROD & ZOOGENET RESOURCES PRESERVAT MADRID 28040 SPAIN
Titolo Testata:
Nature biotechnology
fascicolo: 4, volume: 16, anno: 1998,
pagine: 349 - 354
SICI:
1087-0156(1998)16:4<349:EPITMS>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
MATRIX-ATTACHMENT REGIONS; PROTEIN GENE PROMOTER; WHEY ACIDIC PROTEIN; TRANSMISSIBLE GASTROENTERITIS; IMMUNOGLOBULIN GENES; CHICKEN LYSOZYME; EXPRESSION; CORONAVIRUS; PIGS; IGA;
Keywords:
APPLIED IMMUNOLOGY; ANTIBODY ENGINEERING; MUCOSAL IMMUNITY; CORONAVIRUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
J. Castilla et al., "ENGINEERING PASSIVE-IMMUNITY IN TRANSGENIC MICE SECRETING VIRUS-NEUTRALIZING ANTIBODIES IN MILK", Nature biotechnology, 16(4), 1998, pp. 349-354

Abstract

Protection against enteric infections can be provided by the oral administration of pathogen-neutralizing antibodies. To provide passive immunity, 18 lines of transgenic mice secreting a recombinant monoclonalantibody (Mab) neutralizing transmissible gastroenteritis coronavirus(TGEV) into the milk were generated. The genes encoding a chimeric Mab with the variable modules of the murine TGEV-specific Mab 6A.C3 and the constant modules of a human IgG(1) isotype Mab were expressed under the control of regulatory sequences derived from the whey acidic protein, which is an abundant milk protein. The Mab 6A.C3 binds to a highly conserved epitope present in coronaviruses of several species, which does not allow the selection of neutralization escape mutants. Antibody expression titers of 10(6) were obtained in the milk of transgenicmice that reduced TGEV infectivity 10(6)-fold. The antibody was synthesized at high levels throughout lactation. Integration of matrix attachment region sequences with the antibody genes red to a 20- to 10,000-fold increase in the antibody titer in 50% of the transgenic animals. Antibody expression levels were transgene copy number independent andrelated to the site of integration. The generation of transgenic animals producing virus neutralizing antibodies in milk could provide an approach to protection against neonatal infections of the enteric tract.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 00:49:50