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Titolo:
KSA ANTIGEN EP-CAM MEDIATES CELL-CELL ADHESION OF PANCREATIC EPITHELIAL-CELLS - MORPHOREGULATORY ROLES IN PANCREATIC-ISLET DEVELOPMENT
Autore:
CIRULLI V; CRISA L; BEATTIE GM; MALLY MI; LOPEZ AD; FANNON A; PTASZNIK A; INVERARDI L; RICORDI C; DEERINCK T; ELLISMAN M; REISFELD RA; HAYEK A;
Indirizzi:
UNIV CALIF SAN DIEGO,DEPT PEDIAT,WHITTIER INST DIABET,ISLET RES LABS,9894 GENESEE AVE LA JOLLA CA 92037 UNIV CALIF SAN DIEGO,NATL CTR MICROSCOPY & IMAGING RES LA JOLLA CA 92037 SCRIPPS CLIN & RES INST,DEPT MOL & EXPT MED LA JOLLA CA 92037 SCRIPPS CLIN & RES INST,DEPT IMMUNOL LA JOLLA CA 92037 UNIV MIAMI,SCH MED,DIABET RES INST MIAMI FL 33136 CUNY MT SINAI SCH MED,BROOKDALE CTR MOL BIOL NEW YORK NY 10029
Titolo Testata:
The Journal of cell biology
fascicolo: 6, volume: 140, anno: 1998,
pagine: 1519 - 1534
SICI:
0021-9525(1998)140:6<1519:KAEMCA>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-FETAL PANCREAS; MOLECULE N-CAM; HEPATOCYTE GROWTH-FACTOR; MONOCLONAL-ANTIBODY; ENDOCRINE-CELLS; E-CADHERIN; SURFACE GLYCOPROTEIN; SECRETORY PROTEINS; CARCINOMA MARKER; TRANSGENIC MICE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
96
Recensione:
Indirizzi per estratti:
Citazione:
V. Cirulli et al., "KSA ANTIGEN EP-CAM MEDIATES CELL-CELL ADHESION OF PANCREATIC EPITHELIAL-CELLS - MORPHOREGULATORY ROLES IN PANCREATIC-ISLET DEVELOPMENT", The Journal of cell biology, 140(6), 1998, pp. 1519-1534

Abstract

Cell adhesion molecules (CAMS) are important mediators of cell-cell interactions and regulate cell fate determination by influencing growth, differentiation, and organization within tissues. The human pancarcinoma antigen KSA is a glycoprotein of 40 kD originally identified as amarker of rapidly proliferating tumors of epithelial origin. Interestingly, most normal epithelia also express this antigen, although at lower levels, suggesting that a dynamic regulation of KSA may occur during cell growth and differentiation. Recently, evidence has been provided that this glycoprotein may function as an epithelial cell adhesion molecule (Ep-CAM). Here, we report that Ep-CAM exhibits the features of a morphoregulatory molecule involved in the development of human pancreatic islets. We demonstrate that Ep-CAM expression is targeted to the lateral domain of epithelial cells of the human fetal pancreas, andthat it mediates calcium-independent cell-cell adhesion. Quantitativeconfocal immunofluorescence in fetal pancreata identified the highestlevels of Ep-CAM expression in developing islet-like cell clusters budding from the ductal epithelium, a cell compartment thought to comprise endocrine progenitors. A surprisingly reversed pattern was observedpattern was observed in the human adult pancreas, displaying low levels of Ep-CAM in islet cells and high levels in ducts. We further demonstrate that culture conditions promoting epithelial cell growth induceupregulation of Ep-CAM, whereas endocrine differentiation of fetal pancreatic epithelial cells, transplanted in nude mice, is associated with a downregulation of Ep-CAM expression. In addition, a blockade of Ep-CAM function by KS1/4 mAb induced insulin and glucagon gene transcription and translation in fetal pancreatic cell clusters. These resultsindicate that developmentally regulated expression and function of Ep-CAM play a morphoregulatory role in pancreatic islet ontogeny.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 15:42:39