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Titolo:
SYNTAXIN-12, A MEMBER OF THE SYNTAXIN FAMILY LOCALIZED TO THE ENDOSOME
Autore:
TANG BL; TAN AEH; LIM LK; LEE SS; LOW DYH; HONG WJ;
Indirizzi:
INST MOL & CELL BIOL,MEMBRANE BIOL LAB,30 MED DR SINGAPORE 117609 SINGAPORE
Titolo Testata:
The Journal of biological chemistry
fascicolo: 12, volume: 273, anno: 1998,
pagine: 6944 - 6950
SICI:
0021-9258(1998)273:12<6944:SAMOTS>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANS-GOLGI NETWORK; INTEGRAL MEMBRANE-PROTEIN; BREFELDIN-A; PHOSPHATIDYLINOSITOL 3-KINASE; ORGANELLE STRUCTURE; SECRETORY PATHWAY; FUSION; VESICLES; CELLS; TGN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
B.L. Tang et al., "SYNTAXIN-12, A MEMBER OF THE SYNTAXIN FAMILY LOCALIZED TO THE ENDOSOME", The Journal of biological chemistry, 273(12), 1998, pp. 6944-6950

Abstract

We have cloned a new member of the syntaxin family of proteins, The open reading frame encodes a polypeptide of 272 amino acids with potential coiled-coil domains and a C-terminal hydrophobic tail, Northern blot analysis showed that the transcript is fairly ubiquitous, A solublerecombinant form of the polypeptide without the hydrophobic region binds to alpha-SNAP (soluble N ethylmaleimide-sensitive factor attachment protein) and syndet/SNAP-23 in vitro, Polyclonal antibody raised against the recombinant protein recognized a 39-kDa protein in the membrane fraction of cell lysates, Indirect immunofluorescence studies usingthe polyclonal antibody showed that the protein is localized to intracellular membrane structures, Selective permeabilization studies with digitonin and saponin indicate that the epitope(s) recognized by the antibody is expose to the cytoplasm, consistent with the predicted orientation characteristic of SNAP receptor molecules, Morphological alterations of the staining pattern of the protein with brefeldin A and wortmannin treatment indicate that the protein is localize to the endosome, The cDNA we have cloned apparently corresponded to three previouslydescribed expressed sequence tags named as syntaxins 12, 13, and 14, respectively, We therefore propose to retain the name syntaxin 12 for this protein.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 07:31:23