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Titolo:
DIMERIZATION AND AUTOPROCESSING OF THE NEDD2 (CASPASE-2) PRECURSOR REQUIRES BOTH THE PRODOMAIN AND THE CARBOXYL-TERMINAL REGIONS
Autore:
BUTT AJ; HARVEY NL; PARASIVAM G; KUMAR S;
Indirizzi:
INST MED & VET SCI,HANSON CTR CANC RES,FROME RD,POB 14,RUNDLE MALL ADELAIDE SA 5000 AUSTRALIA INST MED & VET SCI,HANSON CTR CANC RES ADELAIDE SA 5000 AUSTRALIA
Titolo Testata:
The Journal of biological chemistry
fascicolo: 12, volume: 273, anno: 1998,
pagine: 6763 - 6768
SICI:
0021-9258(1998)273:12<6763:DAAOTN>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
ICE-LIKE PROTEASES; DEATH GENE CED-3; CELL-DEATH; INTERLEUKIN-1-BETA-CONVERTING ENZYME; APOPTOSIS; EXPRESSION; ENCODES; SELECTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
A.J. Butt et al., "DIMERIZATION AND AUTOPROCESSING OF THE NEDD2 (CASPASE-2) PRECURSOR REQUIRES BOTH THE PRODOMAIN AND THE CARBOXYL-TERMINAL REGIONS", The Journal of biological chemistry, 273(12), 1998, pp. 6763-6768

Abstract

Nedd2 (caspase-2) is a cysteine protease of the caspase family that has been demonstrated to play a role in the apoptotic pathway. The 51-kDa precursor of Nedd2 undergoes cleavage into two subunits following various apoptotic stimuli. In this study, we have investigated the dimerization of the Nedd2 precursor (pro-Nedd2) in Saccharomyces cerevisiae and its self-processing activity in vivo. We demonstrate that the expression of pro-Nedd2 in yeast cells results in processing of the precursor. A catalytically inactive pro-Nedd2 mutant dimerized in yeast, and the dimerization required both the prodomain and the carboxyl-terminal residues. Aspartate mutants that block the removal of the p14/p12 subunits, but not the wild-type Nedd2, were shown to dimerize in yeastcells, suggesting that dimerization occurs prior to processing. In vitro processing of pro-Nedd2 by recombinant active Nedd2 defined the aspartate residues that are crucial for processing to occur. Both the invivo and in vitro processing of pro-Nedd2 directly correlated with its ability to induce cell death in transient overexpression experiments.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 18:54:33